Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS12540 and BPHYT_RS12545 are separated by 12 nucleotidesBPHYT_RS12545 and BPHYT_RS12550 are separated by 49 nucleotidesBPHYT_RS12550 and BPHYT_RS12555 are separated by 72 nucleotidesBPHYT_RS12555 and BPHYT_RS12560 are separated by 153 nucleotides BPHYT_RS12540: BPHYT_RS12540 - membrane protein, at 2,862,533 to 2,863,435 _RS12540 BPHYT_RS12545: BPHYT_RS12545 - membrane protein, at 2,863,448 to 2,864,848 _RS12545 BPHYT_RS12550: BPHYT_RS12550 - GTP-binding protein HflX, at 2,864,898 to 2,866,070 _RS12550 BPHYT_RS12555: BPHYT_RS12555 - RNA-binding protein Hfq, at 2,866,143 to 2,866,379 _RS12555 BPHYT_RS12560: BPHYT_RS12560 - GTPase Der, at 2,866,533 to 2,867,870 _RS12560
Group Condition BPHYT_RS12540 BPHYT_RS12545 BPHYT_RS12550 BPHYT_RS12555 BPHYT_RS12560
carbon source 2-Deoxy-D-ribonic lithium salt (C) N.D. N.D. -4.0 N.D. N.D.
phage P. phyto PHYTO2 MOI 1 N.D. N.D. -3.3 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. N.D. -2.8 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. -2.8 N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB N.D. N.D. -2.6 N.D. N.D.
carbon source 2-Deoxy-D-Ribose (C) N.D. N.D. -2.5 N.D. N.D.
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) N.D. N.D. -2.4 N.D. N.D.
phage 36hr PHYTO3 MOI 1 N.D. N.D. -2.3 N.D. N.D.
nitrogen source L-Isoleucine (N) N.D. N.D. -2.2 N.D. N.D.
phage 36hr PHYTO3 MOI 0.1 N.D. N.D. -2.2 N.D. N.D.
phage 36hr PHYTO11 MOI 1 N.D. N.D. -2.2 N.D. N.D.
phage 36hr PHYTO3 MOI 1 N.D. N.D. -2.2 N.D. N.D.
phage 24hr PHYTO3 MOI 1 N.D. N.D. -2.2 N.D. N.D.
carbon source Malonic (C) N.D. N.D. -2.1 N.D. N.D.
phage 24hr PHYTO2 MOI 0.1 N.D. N.D. -2.1 N.D. N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. N.D. -1.9 N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. N.D. -1.8 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. N.D. -1.8 N.D. N.D.
vitamin No vitamins t1 N.D. N.D. -1.7 N.D. N.D.
phage 36hr PHYTO8 MOI 0.1 N.D. N.D. +1.7 N.D. N.D.
phage 24hr PHYTO4 MOI 0.1 N.D. N.D. +1.8 N.D. N.D.
phage 24hr PHYTO2 MOI 1 N.D. N.D. +1.8 N.D. N.D.
phage 24hr PHYTO6 MOI 1 N.D. N.D. +1.8 N.D. N.D.
phage 24hr PHYTO2 MOI 0.1 N.D. N.D. +1.9 N.D. N.D.
phage 36hr PHYTO8 MOI 10 N.D. N.D. +2.1 N.D. N.D.
phage 24hr PHYTO3 MOI 0.1 N.D. N.D. +2.2 N.D. N.D.
phage 36hr PHYTO6 MOI 10 N.D. N.D. +2.2 N.D. N.D.
phage 36hr PHYTO11 MOI 1 N.D. N.D. +2.2 N.D. N.D.
motility outer cut, LB soft agar motility assay N.D. N.D. +2.4 N.D. N.D.
nophagecontrol 36hr Phyto Lib only N.D. N.D. +2.4 N.D. N.D.
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