Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS12495 and BPHYT_RS12500 are separated by 64 nucleotidesBPHYT_RS12500 and BPHYT_RS12505 are separated by 127 nucleotidesBPHYT_RS12505 and BPHYT_RS12510 are separated by 357 nucleotidesBPHYT_RS12510 and BPHYT_RS12515 are separated by 187 nucleotides BPHYT_RS12495: BPHYT_RS12495 - helicase, at 2,850,040 to 2,852,307 _RS12495 BPHYT_RS12500: BPHYT_RS12500 - hypothetical protein, at 2,852,372 to 2,852,590 _RS12500 BPHYT_RS12505: BPHYT_RS12505 - lipoprotein, at 2,852,718 to 2,853,647 _RS12505 BPHYT_RS12510: BPHYT_RS12510 - RNA-binding protein, at 2,854,005 to 2,856,353 _RS12510 BPHYT_RS12515: BPHYT_RS12515 - potassium transporter Kup, at 2,856,541 to 2,858,427 _RS12515
Group Condition BPHYT_RS12495 BPHYT_RS12500 BPHYT_RS12505 BPHYT_RS12510 BPHYT_RS12515
phage P. phyto corn MOI 0.1 -0.1 -0.3 N.D. -3.9 N.D.
phage 36hr PHYTO11 MOI 0.1 +0.2 -1.4 N.D. -3.0 N.D.
phage 36hr PHYTO9 MOI 0.1 -0.0 -0.9 N.D. -3.2 N.D.
phage 24hr PHYTO6 MOI 0.1 +0.0 -1.0 N.D. -3.0 N.D.
phage 36hr PHYTO4 MOI 1 +0.0 -1.7 N.D. -2.3 N.D.
phage 24hr PHYTO8 MOI 1 -0.1 -0.9 N.D. -2.6 N.D.
nophagecontrol 36hr Phyto Lib only +0.0 -0.7 N.D. -2.9 N.D.
nophagecontrol 36hr Phyto Lib only -0.2 -0.5 N.D. -2.9 N.D.
phage 36hr PHYTO6 MOI 1 -0.2 -1.6 N.D. -1.5 N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 -0.5 N.D. -2.9 N.D.
phage 24hr PHYTO2 MOI 0.1 -0.1 -0.0 N.D. -3.1 N.D.
phage 24hr PHYTO9 MOI 0.1 +0.1 -0.5 N.D. -2.8 N.D.
phage 24hr PHYTO11 MOI 1 +0.0 -0.6 N.D. -2.6 N.D.
nophagecontrol 36hr Phyto Lib Only -0.3 +0.2 N.D. -2.9 N.D.
phage 36hr PHYTO2 MOI 1 +0.2 -0.6 N.D. -2.7 N.D.
phage 36hr PHYTO11 MOI 1 +0.0 +0.0 N.D. -3.1 N.D.
motility outer cut, LB soft agar motility assay -0.2 -0.4 N.D. -2.3 N.D.
phage 36hr PHYTO11 MOI 1 -0.1 +0.2 N.D. -3.0 N.D.
phage 36hr PHYTO1 MOI 1 +0.1 -0.0 N.D. -2.9 N.D.
phage 24hr PHYTO2 MOI 1 +0.1 +0.3 N.D. -3.1 N.D.
nophagecontrol 24hr Phyto Lib Only +0.0 +0.5 N.D. -3.1 N.D.
motility outer cut, LB soft agar motility assay -0.1 +0.1 N.D. -2.5 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.0 +0.3 N.D. -2.9 N.D.
phage P. phyto PHYTO2 MOI 0.1 +0.2 +0.7 N.D. -3.4 N.D.
phage 36hr PHYTO4 MOI 1 +0.3 -0.2 N.D. -2.5 N.D.
phage 36hr PHYTO2 MOI 1 -0.1 -2.3 N.D. +0.1 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.3 -0.2 N.D. -2.3 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.7 +0.2 N.D. -1.6 N.D.
phage P. phyto PHYTO2 MOI 1 +0.2 +0.2 N.D. -2.1 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.0 +0.6 N.D. -2.1 N.D.
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