Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS12455 and BPHYT_RS12460 are separated by 208 nucleotidesBPHYT_RS12460 and BPHYT_RS12465 are separated by 398 nucleotidesBPHYT_RS12465 and BPHYT_RS12470 are separated by 201 nucleotidesBPHYT_RS12470 and BPHYT_RS12475 are separated by 157 nucleotides BPHYT_RS12455: BPHYT_RS12455 - serine O-acetyltransferase, at 2,838,815 to 2,839,651 _RS12455 BPHYT_RS12460: BPHYT_RS12460 - RNA methyltransferase, at 2,839,860 to 2,840,693 _RS12460 BPHYT_RS12465: BPHYT_RS12465 - inositol monophosphatase, at 2,841,092 to 2,841,895 _RS12465 BPHYT_RS12470: BPHYT_RS12470 - major facilitator transporter, at 2,842,097 to 2,843,395 _RS12470 BPHYT_RS12475: BPHYT_RS12475 - DNA mismatch repair protein MutS, at 2,843,553 to 2,846,237 _RS12475
Group Condition BPHYT_RS12455 BPHYT_RS12460 BPHYT_RS12465 BPHYT_RS12470 BPHYT_RS12475
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -0.5 -0.2 -5.3 -0.1 +0.3
carbon source Putrescine (C) +0.0 -0.0 -5.2 -0.1 -0.1
nutrient L-Histidine -0.1 -0.1 -4.4 +0.0 -0.2
motility inner cut, LB soft agar motility assay -0.3 -0.3 -3.5 +0.3 -0.7
temperature LB, 23C -0.1 +0.1 -4.4 +0.1 -0.1
carbon source Azelaic (C) -0.6 -0.2 -4.0 +0.2 +0.2
carbon source L-Proline (C) -0.7 +0.0 -3.6 -0.0 -0.1
supernatant control Vogels_fungal_media; pH 5.5 -0.1 -0.2 -3.5 -0.0 -0.5
in planta Plant=Zea_mays; PlantTreatment=None; sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_R2A_with_Tween_and_Cycloheximide; Time=15_days -0.2 -0.2 -3.9 +0.1 -0.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB -1.1 -0.3 -2.4 -0.2 -0.2
nutrient L-Serine -0.2 -0.0 -3.9 +0.1 -0.1
motility outer cut, LB soft agar motility assay -0.4 +0.4 -3.7 -0.0 -0.3
vitamin With vitamins t2 +0.1 -0.2 -3.7 -0.1 -0.1
vitamin No vitamins t2 -0.3 -0.0 -3.6 +0.1 +0.1
carbon source Protocatechuic Acid (C) -0.3 -0.4 -3.2 +0.0 +0.2
motility outer cut, LB soft agar motility assay -0.4 -0.2 -3.5 +0.5 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.5 -0.4 -3.5 -0.2 +0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 +0.1 -3.6 -0.2 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 +0.2 -3.8 +0.1 +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 +0.3 -3.4 +0.2 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.0 +0.5 -3.5 +0.1 +0.2
nitrogen source Ammonium chloride (N) +0.4 +0.2 -3.3 +0.1 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.7 +0.3 -3.1 -0.1 -0.2
tn7 efficiency Outgrowth after Tn7 transformation +0.5 +0.3 -3.3 +0.0 +0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.4 +0.5 -3.5 -0.0 +0.3
motility inner cut, LB soft agar motility assay +0.7 +0.4 -2.9 -0.0 -0.4
stress Nickel (II) chloride 1 mM +1.7 +0.2 -3.5 -0.0 -0.1
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 -0.1 -0.4 -2.9 -0.3 +4.4
phage 24hr PHYTO3 MOI 10 +0.8 +0.0 +3.3 -0.4 +0.4
stress Paraquat dichloride 0.064 mg/ml +0.2 +0.1 +4.4 +0.0 -0.3
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