Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS12360 and BPHYT_RS12365 are separated by 127 nucleotidesBPHYT_RS12365 and BPHYT_RS12370 are separated by 137 nucleotidesBPHYT_RS12370 and BPHYT_RS12375 overlap by 4 nucleotidesBPHYT_RS12375 and BPHYT_RS12380 overlap by 1 nucleotides BPHYT_RS12360: BPHYT_RS12360 - esterase, at 2,817,633 to 2,818,586 _RS12360 BPHYT_RS12365: BPHYT_RS12365 - peptidase P60, at 2,818,714 to 2,819,379 _RS12365 BPHYT_RS12370: BPHYT_RS12370 - ABC transporter, at 2,819,517 to 2,821,151 _RS12370 BPHYT_RS12375: BPHYT_RS12375 - peptide ABC transporter permease, at 2,821,148 to 2,822,254 _RS12375 BPHYT_RS12380: BPHYT_RS12380 - ABC transporter permease, at 2,822,254 to 2,823,291 _RS12380
Group Condition BPHYT_RS12360 BPHYT_RS12365 BPHYT_RS12370 BPHYT_RS12375 BPHYT_RS12380
motility inner cut, LB soft agar motility assay -0.1 N.D. -0.4 -0.7 -1.3
phage 24hr PHYTO3 MOI 10 -0.1 N.D. -0.3 +0.1 -2.2
phage PHYTO3 MOI 0.1 -0.2 N.D. -0.8 -0.5 -1.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.2 N.D. -0.4 -0.5 -1.2
temperature LB, 37C +0.1 N.D. -0.5 -1.1 -0.6
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 N.D. -0.2 -0.4 -1.8
phage P. phyto PHYTO2 MOI 1 -0.1 N.D. -0.5 -0.4 -1.0
motility outer cut, LB soft agar motility assay +0.1 N.D. -0.4 -0.6 -1.1
phage 24hr PHYTO2 MOI 1 +0.3 N.D. -0.3 +0.0 -2.0
motility outer cut, LB soft agar motility assay -0.0 N.D. -0.5 -0.3 -1.2
motility outer cut, LB soft agar motility assay -0.1 N.D. -0.8 -0.8 -0.3
phage P. phyto PHYTO2 MOI 1 -0.3 N.D. -0.3 -0.4 -0.9
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.0 N.D. -0.1 -0.2 -1.5
nophagecontrol 36hr Phyto Lib Only +0.1 N.D. -0.1 -0.3 -1.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.4 N.D. -0.4 -0.4 -0.6
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 N.D. -0.7 +0.1 -1.1
phage 36hr PHYTO10 MOI 1 +0.2 N.D. -0.4 -0.5 -1.0
phage 36hr PHYTO8 MOI 0.1 +0.2 N.D. +0.1 -0.4 -1.5
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 N.D. -0.1 -0.2 -1.4
phage 24hr PHYTO2 MOI 0.1 -0.1 N.D. -0.0 +0.3 -1.3
phage 36hr PHYTO1 MOI 10 +0.2 N.D. +0.2 -0.4 -1.0
nophagecontrol 36hr Phyto Lib Only +0.4 N.D. -0.4 -0.3 -0.7
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 N.D. +0.1 -0.8 -0.6
phage 24hr PHYTO MOI 0.1 +0.2 N.D. +0.3 -0.4 -1.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.4 N.D. -0.2 +0.3 -0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.6 N.D. -0.4 +0.2 +0.8
phage 24hr PHYTO7 MOI 10 -0.0 N.D. +0.3 +0.6 +1.0
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.1 N.D. +0.5 -0.0 +2.8
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.2 N.D. +0.8 +0.1 +2.4
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.5 N.D. +0.7 -0.1 +3.0
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