Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS12350 and BPHYT_RS12355 are separated by 122 nucleotidesBPHYT_RS12355 and BPHYT_RS12360 are separated by 406 nucleotidesBPHYT_RS12360 and BPHYT_RS12365 are separated by 127 nucleotidesBPHYT_RS12365 and BPHYT_RS12370 are separated by 137 nucleotides BPHYT_RS12350: BPHYT_RS12350 - tRNA-Ala, at 2,815,619 to 2,815,694 _RS12350 BPHYT_RS12355: BPHYT_RS12355 - glutamate--tRNA ligase, at 2,815,817 to 2,817,226 _RS12355 BPHYT_RS12360: BPHYT_RS12360 - esterase, at 2,817,633 to 2,818,586 _RS12360 BPHYT_RS12365: BPHYT_RS12365 - peptidase P60, at 2,818,714 to 2,819,379 _RS12365 BPHYT_RS12370: BPHYT_RS12370 - ABC transporter, at 2,819,517 to 2,821,151 _RS12370
Group Condition BPHYT_RS12350 BPHYT_RS12355 BPHYT_RS12360 BPHYT_RS12365 BPHYT_RS12370
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 N.D. N.D. -0.2 N.D. -1.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days N.D. N.D. -0.4 N.D. -0.6
phage PHYTO3 MOI 0.1 N.D. N.D. -0.2 N.D. -0.8
motility outer cut, LB soft agar motility assay N.D. N.D. -0.1 N.D. -0.8
carbon source Tween 20 (C) N.D. N.D. +0.2 N.D. -1.0
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. -0.1 N.D. -0.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days N.D. N.D. -0.3 N.D. -0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days N.D. N.D. -0.3 N.D. -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. -0.4 N.D. -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. N.D. -0.4 N.D. -0.4
stress Bacitracin 0.25 mg/ml N.D. N.D. -0.2 N.D. -0.5
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 N.D. N.D. -0.2 N.D. -0.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB N.D. N.D. -0.2 N.D. -0.5
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent N.D. N.D. +0.2 N.D. -0.5
phage 36hr PHYTO10 MOI 1 N.D. N.D. +0.2 N.D. -0.4
phage 24hr PHYTO4 MOI 0.1 N.D. N.D. +0.3 N.D. -0.5
phage 36hr PHYTO6 MOI 10 N.D. N.D. +0.3 N.D. -0.5
phage 36hr PHYTO6 MOI 1 N.D. N.D. +0.3 N.D. -0.5
nophagecontrol 36hr Phyto Lib Only N.D. N.D. +0.4 N.D. -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. N.D. +0.6 N.D. -0.4
nitrogen source L-Serine (N) N.D. N.D. +0.5 N.D. -0.2
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 N.D. N.D. +0.6 N.D. -0.2
nitrogen source NAG (N) N.D. N.D. +0.4 N.D. +0.3
nitrogen source L-Glutamic (N) N.D. N.D. +0.8 N.D. -0.1
phage 36hr PHYTO7 MOI 1 N.D. N.D. +0.2 N.D. +0.4
phage 24hr PHYTO10 MOI 10 N.D. N.D. +0.3 N.D. +0.5
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. +0.5 N.D. +0.2
carbon source D-Glucose 20 mM (C) N.D. N.D. +0.3 N.D. +0.5
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. +0.2 N.D. +0.8
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. +0.5 N.D. +0.7
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