Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS12295 and BPHYT_RS12300 are separated by 151 nucleotidesBPHYT_RS12300 and BPHYT_RS12305 are separated by 83 nucleotidesBPHYT_RS12305 and BPHYT_RS12310 are separated by 174 nucleotidesBPHYT_RS12310 and BPHYT_RS12315 are separated by 94 nucleotides BPHYT_RS12295: BPHYT_RS12295 - LysR family transcriptional regulator, at 2,808,325 to 2,809,227 _RS12295 BPHYT_RS12300: BPHYT_RS12300 - haloacid dehalogenase, at 2,809,379 to 2,810,194 _RS12300 BPHYT_RS12305: BPHYT_RS12305 - Malate synthase (EC 2.3.3.9) (from data), at 2,810,278 to 2,811,888 _RS12305 BPHYT_RS12310: BPHYT_RS12310 - gamma-glutamyl cyclotransferase, at 2,812,063 to 2,812,464 _RS12310 BPHYT_RS12315: BPHYT_RS12315 - ribonuclease, at 2,812,559 to 2,813,053 _RS12315
Group Condition BPHYT_RS12295 BPHYT_RS12300 BPHYT_RS12305 BPHYT_RS12310 BPHYT_RS12315
carbon source Tween 20 (C) -2.3 -1.3 -2.6 N.D. N.D.
phage 36hr PHYTO4 MOI 1 -1.4 -0.2 -0.5 N.D. N.D.
nophagecontrol 24hr Phyto Lib Only -0.8 -0.3 -0.6 N.D. N.D.
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -0.7 -0.5 -0.5 N.D. N.D.
phage PHYTO3 MOI 0.1 -1.6 +0.2 -0.1 N.D. N.D.
phage 36hr PHYTO4 MOI 1 -1.4 +0.1 -0.2 N.D. N.D.
phage 24hr PHYTO6 MOI 10 -1.1 -0.1 -0.3 N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB -0.8 -0.5 -0.1 N.D. N.D.
phage 36hr PHYTO10 MOI 10 -1.2 +0.0 -0.3 N.D. N.D.
phage 36hr PHYTO2 MOI 0.1 -1.2 -0.1 -0.1 N.D. N.D.
phage 36hr PHYTO9 MOI 1 -1.4 +0.2 -0.2 N.D. N.D.
motility inner cut, LB soft agar motility assay -1.5 +0.1 +0.0 N.D. N.D.
phage 36hr PHYTO1 MOI 10 -1.3 +0.3 -0.3 N.D. N.D.
phage 36hr PHYTO10 MOI 10 -1.8 +0.4 +0.0 N.D. N.D.
phage 36hr PHYTO11 MOI 1 -1.6 +0.4 -0.1 N.D. N.D.
phage 24hr PHYTO6 MOI 0.1 -0.9 +0.0 -0.4 N.D. N.D.
phage 24hr PHYTO2 MOI 0.1 -1.3 +0.1 +0.0 N.D. N.D.
nophagecontrol 24hr Phyto Lib Only -1.0 +0.1 -0.3 N.D. N.D.
phage 24hr PHYTO8 MOI 1 -1.6 +0.6 -0.2 N.D. N.D.
phage 36hr PHYTO11 MOI 0.1 -1.2 +0.2 -0.2 N.D. N.D.
phage 36hr PHYTO7 MOI 1 -1.3 +0.2 -0.0 N.D. N.D.
phage 36hr PHYTO3 MOI 1 -0.9 +0.2 -0.4 N.D. N.D.
phage P. phyto PHYTO2 MOI 1 -1.2 +0.3 -0.0 N.D. N.D.
phage 36hr PHYTO6 MOI 0.1 -0.9 +0.3 -0.2 N.D. N.D.
carbon source Dodecandioic (C) -1.1 +0.0 +0.2 N.D. N.D.
carbon source adipate (C) -0.6 +0.9 +0.0 N.D. N.D.
carbon source Malonic (C) +0.9 +0.6 -0.1 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +1.5 +0.2 -0.1 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +1.9 -0.2 -0.1 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +1.1 +0.4 +0.6 N.D. N.D.
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