Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS12255 and BPHYT_RS12260 overlap by 4 nucleotidesBPHYT_RS12260 and BPHYT_RS12265 overlap by 14 nucleotidesBPHYT_RS12265 and BPHYT_RS12270 overlap by 4 nucleotidesBPHYT_RS12270 and BPHYT_RS12275 are separated by 560 nucleotides BPHYT_RS12255: BPHYT_RS12255 - hypothetical protein, at 2,799,147 to 2,800,160 _RS12255 BPHYT_RS12260: BPHYT_RS12260 - DNA polymerase, at 2,800,157 to 2,801,401 _RS12260 BPHYT_RS12265: BPHYT_RS12265 - alanine acetyltransferase, at 2,801,388 to 2,801,882 _RS12265 BPHYT_RS12270: BPHYT_RS12270 - O-sialoglycoprotein endopeptidase, at 2,801,879 to 2,802,709 _RS12270 BPHYT_RS12275: BPHYT_RS12275 - acyl-CoA-binding protein, at 2,803,270 to 2,803,539 _RS12275
Group Condition BPHYT_RS12255 BPHYT_RS12260 BPHYT_RS12265 BPHYT_RS12270 BPHYT_RS12275
lb_nosalt LB_noSalt -0.2 N.D. -2.3 N.D. -0.3
phage 24hr PHYTO3 MOI 10 -0.3 N.D. -2.4 N.D. +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 N.D. -1.8 N.D. -0.2
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.1 N.D. -2.4 N.D. +0.4
nitrogen source L-Lysine (N) -0.2 N.D. -1.7 N.D. -0.1
phage P. phyto PHYTO2 MOI 0.1 +0.1 N.D. -2.3 N.D. +0.2
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.4 N.D. -1.7 N.D. +0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB -0.2 N.D. -2.0 N.D. +0.4
lb_dap plate control LB_DAP agar plate, outgrowth in LB -0.2 N.D. -1.4 N.D. -0.2
carbon source D-Glucose (C) -0.2 N.D. -1.6 N.D. +0.0
no phage control No phage control +0.1 N.D. -1.5 N.D. -0.2
lb_dap plate control LB_DAP agar plate, outgrowth in LB -0.1 N.D. -1.4 N.D. -0.1
phage 24hr PHYTO MOI 0.1 +0.1 N.D. -1.5 N.D. -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.3 N.D. -1.3 N.D. +0.3
nophagecontrol 24hr Phyto Lib Only +0.4 N.D. -1.6 N.D. +0.0
phage 24hr PHYTO2 MOI 0.1 +0.4 N.D. -1.3 N.D. -0.1
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.0 N.D. -1.4 N.D. +0.2
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) -0.2 N.D. -1.4 N.D. +0.5
phage 36hr PHYTO11 MOI 0.1 +0.1 N.D. -1.3 N.D. +0.2
phage 36hr PHYTO6 MOI 0.1 +0.5 N.D. -1.5 N.D. +0.0
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.5 N.D. -2.0 N.D. +0.5
phage 36hr PHYTO2 MOI 1 +0.3 N.D. -1.4 N.D. +0.2
carbon source 2-Deoxy-D-Ribose (C) +0.1 N.D. -1.3 N.D. +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.5 N.D. +1.1 N.D. +0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.3 N.D. +1.2 N.D. +0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.3 N.D. +1.3 N.D. +0.4
stress Paraquat dichloride 0.032 mg/ml +0.0 N.D. +1.8 N.D. +0.1
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent -0.1 N.D. +1.4 N.D. +0.7
carbon source Suberic (C) +0.1 N.D. +1.5 N.D. +0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 N.D. +2.3 N.D. +0.0
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