Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS12225 and BPHYT_RS12230 are separated by 222 nucleotidesBPHYT_RS12230 and BPHYT_RS12235 are separated by 143 nucleotidesBPHYT_RS12235 and BPHYT_RS12240 are separated by 90 nucleotidesBPHYT_RS12240 and BPHYT_RS12245 are separated by 221 nucleotides BPHYT_RS12225: BPHYT_RS12225 - carnitine dehydratase, at 2,792,280 to 2,793,323 _RS12225 BPHYT_RS12230: BPHYT_RS12230 - hypothetical protein, at 2,793,546 to 2,793,824 _RS12230 BPHYT_RS12235: BPHYT_RS12235 - DNA repair protein RadA, at 2,793,968 to 2,795,344 _RS12235 BPHYT_RS12240: BPHYT_RS12240 - alanine racemase, at 2,795,435 to 2,796,505 _RS12240 BPHYT_RS12245: BPHYT_RS12245 - major facilitator transporter, at 2,796,727 to 2,798,034 _RS12245
Group Condition BPHYT_RS12225 BPHYT_RS12230 BPHYT_RS12235 BPHYT_RS12240 BPHYT_RS12245
stress Lomefloxacin 0.001 mM -0.2 -0.1 -2.2 N.D. N.D.
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -0.4 -1.0 -1.0 N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB -1.3 -0.7 -0.2 N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -1.4 +0.0 -0.7 N.D. N.D.
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 +0.8 -0.4 -2.3 N.D. N.D.
phage 24hr PHYTO9 MOI 0.1 -0.8 +0.2 -1.1 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.5 -0.4 -0.7 N.D. N.D.
phage 36hr PHYTO10 MOI 10 -1.5 +0.0 +0.0 N.D. N.D.
nitrogen source L-Lysine (N) -0.4 -0.1 -0.9 N.D. N.D.
phage 36hr PHYTO1 MOI 1 -1.1 +0.3 -0.5 N.D. N.D.
motility inner cut, LB soft agar motility assay -0.9 +0.3 -0.6 N.D. N.D.
nophagecontrol 24hr Phyto Lib only -1.1 +0.7 -0.6 N.D. N.D.
phage 24hr PHYTO11 MOI 1 -1.1 +0.3 -0.1 N.D. N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.8 +0.5 -0.3 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.4 +0.6 -0.6 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.8 +0.3 +0.3 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.4 +0.7 -0.4 N.D. N.D.
carbon source Suberic (C) +0.4 -0.8 +0.3 N.D. N.D.
phage 24hr PHYTO7 MOI 1 -0.4 +0.8 -0.3 N.D. N.D.
temperature LB, 37C +0.1 +0.8 -0.6 N.D. N.D.
nitrogen source NAG (N) +0.4 -0.4 +0.6 N.D. N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 +0.4 +0.7 -0.5 N.D. N.D.
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X -0.2 +0.9 +0.2 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 -0.3 +0.9 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.7 +0.7 -0.1 N.D. N.D.
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 +0.6 +0.2 +0.8 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.0 +1.3 +0.3 N.D. N.D.
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days +0.4 +0.3 +0.9 N.D. N.D.
phage 24hr PHYTO3 MOI 10 +0.6 +0.7 +0.5 N.D. N.D.
phage 36hr PHYTO10 MOI 10 +0.3 +1.5 +0.1 N.D. N.D.
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