Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS12205 and BPHYT_RS12210 are separated by 392 nucleotidesBPHYT_RS12210 and BPHYT_RS12215 are separated by 11 nucleotidesBPHYT_RS12215 and BPHYT_RS12220 are separated by 134 nucleotidesBPHYT_RS12220 and BPHYT_RS12225 are separated by 343 nucleotides BPHYT_RS12205: BPHYT_RS12205 - N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase, at 2,786,630 to 2,787,514 _RS12205 BPHYT_RS12210: BPHYT_RS12210 - cardiolipin synthetase, at 2,787,907 to 2,789,358 _RS12210 BPHYT_RS12215: BPHYT_RS12215 - glutathione peroxidase, at 2,789,370 to 2,789,849 _RS12215 BPHYT_RS12220: BPHYT_RS12220 - glutathione ABC transporter ATP-binding protein, at 2,789,984 to 2,791,936 _RS12220 BPHYT_RS12225: BPHYT_RS12225 - carnitine dehydratase, at 2,792,280 to 2,793,323 _RS12225
Group Condition BPHYT_RS12205 BPHYT_RS12210 BPHYT_RS12215 BPHYT_RS12220 BPHYT_RS12225
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.1 -0.6 -0.6 +0.1 -1.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB -0.7 -0.2 +0.1 -0.3 -1.3
phage 36hr PHYTO10 MOI 10 -0.6 -0.1 +0.0 +0.2 -1.5
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days +0.1 -0.5 -0.8 -0.2 -0.4
carbon source Phenylacetic 5 mM (C) -0.7 -0.4 -0.5 +0.3 +0.0
supernatant control Vogels_fungal_media 0.1X -0.8 +0.1 -0.4 -0.4 +0.3
carbon source D-Glucose 20 mM (C) -0.6 -0.0 -0.4 +0.4 -0.4
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) -0.7 -0.3 -0.3 -0.1 +0.5
phage 24hr PHYTO9 MOI 0.1 -0.1 -0.2 +0.5 -0.3 -0.8
carbon source Phenylacetic 5 mM (C) -0.9 -0.2 -0.4 +0.4 +0.5
motility inner cut, LB soft agar motility assay -0.3 -0.2 +0.4 +0.5 -0.9
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB -0.3 -0.7 +0.8 +0.1 -0.4
phage 36hr PHYTO2 MOI 1 -0.5 -0.6 +0.5 -0.1 +0.3
phage 24hr PHYTO6 MOI 10 +0.2 +0.1 +0.4 +0.1 -1.1
phage 24hr PHYTO11 MOI 1 -0.1 +0.1 +0.5 +0.4 -1.1
nophagecontrol 24hr Phyto Lib only +0.5 -0.0 +0.6 +0.1 -1.1
in planta Plant=Brachypodium_distachyon ; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.3 -0.7 +0.1 -0.2 +0.5
supernatant control Vogels_fungal_media; pH 5.5 +0.4 -0.7 +0.6 -0.2 -0.0
phage 24hr PHYTO9 MOI 10 +0.2 -0.2 +0.7 +0.3 -0.7
in planta Plant=Brachypodium_distachyon ; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.3 -0.5 +0.4 -0.4 +0.7
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 -0.0 +0.2 +0.5 -0.9 +0.8
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.3 +0.1 +0.7 +0.5 -0.4
phage 36hr PHYTO MOI 0.1 -0.6 +0.3 +0.3 +0.3 +0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.7 +0.1 +0.8 -0.3 -0.1
phage 24hr PHYTO4 MOI 10 +0.5 -0.5 +0.4 +0.4 +0.4
phage 36hr PHYTO3 MOI 1 -0.3 +0.2 +0.4 +0.5 +0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +1.5 +0.0 -0.1 +0.3 -0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +1.2 +0.2 -0.3 +0.4 +0.1
phage 36hr PHYTO8 MOI 0.1 +0.6 -0.3 +0.6 -0.0 +0.9
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +1.0 +0.2 +0.4 +0.1 +0.4
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