Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS12180 and BPHYT_RS12185 are separated by 682 nucleotidesBPHYT_RS12185 and BPHYT_RS12190 are separated by 70 nucleotidesBPHYT_RS12190 and BPHYT_RS12195 overlap by 1 nucleotidesBPHYT_RS12195 and BPHYT_RS12200 are separated by 243 nucleotides BPHYT_RS12180: BPHYT_RS12180 - multidrug DMT transporter permease, at 2,781,072 to 2,782,067 _RS12180 BPHYT_RS12185: BPHYT_RS12185 - succinyldiaminopimelate aminotransferase, at 2,782,750 to 2,783,967 _RS12185 BPHYT_RS12190: BPHYT_RS12190 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase, at 2,784,038 to 2,784,865 _RS12190 BPHYT_RS12195: BPHYT_RS12195 - arsenate reductase, at 2,784,865 to 2,785,230 _RS12195 BPHYT_RS12200: BPHYT_RS12200 - succinyl-diaminopimelate desuccinylase, at 2,785,474 to 2,786,613 _RS12200
Group Condition BPHYT_RS12180 BPHYT_RS12185 BPHYT_RS12190 BPHYT_RS12195 BPHYT_RS12200
nitrogen source L-Asparagine (N) +0.1 -1.4 N.D. N.D. N.D.
nitrogen source L-Alanine (N) -0.2 -1.0 N.D. N.D. N.D.
vitamin With vitamins t2 -0.0 -1.0 N.D. N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X -0.4 -0.6 N.D. N.D. N.D.
nitrogen source D-Alanine (N) -0.3 -0.6 N.D. N.D. N.D.
nitrogen source NAG (N) +0.0 -1.0 N.D. N.D. N.D.
carbon source Phenylacetic 5 mM (C) -0.1 -0.9 N.D. N.D. N.D.
nitrogen source Ammonium chloride (N) -0.1 -0.9 N.D. N.D. N.D.
nitrogen source Putrescine (N) -0.3 -0.5 N.D. N.D. N.D.
vitamin No vitamins t2 -0.1 -0.7 N.D. N.D. N.D.
supernatant control Vogels_fungal_media; pH 5.5 +0.1 -0.9 N.D. N.D. N.D.
nitrogen source L-Glutamic (N) +0.0 -0.8 N.D. N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.1 -0.8 N.D. N.D. N.D.
carbon source D-Galactose (C) +0.1 -0.8 N.D. N.D. N.D.
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days +0.4 -0.8 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 -0.6 N.D. N.D. N.D.
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) -0.5 +0.4 N.D. N.D. N.D.
phage P. phyto PHYTO2 MOI 1 -0.3 +0.6 N.D. N.D. N.D.
phage 36hr PHYTO3 MOI 0.1 +0.4 +0.4 N.D. N.D. N.D.
nophagecontrol 24hr Phyto Lib Only +0.4 +0.4 N.D. N.D. N.D.
phage 24hr PHYTO3 MOI 0.1 +0.3 +0.5 N.D. N.D. N.D.
phage 36hr PHYTO10 MOI 10 +0.2 +0.6 N.D. N.D. N.D.
phage 36hr PHYTO8 MOI 10 +0.3 +0.6 N.D. N.D. N.D.
phage 36hr PHYTO6 MOI 10 +0.3 +0.5 N.D. N.D. N.D.
phage 24hr PHYTO10 MOI 10 +0.2 +0.7 N.D. N.D. N.D.
phage 36hr PHYTO3 MOI 1 +0.2 +0.7 N.D. N.D. N.D.
phage 36hr PHYTO11 MOI 1 +0.5 +0.5 N.D. N.D. N.D.
phage 24hr PHYTO11 MOI 1 +0.6 +0.6 N.D. N.D. N.D.
phage 36hr PHYTO3 MOI 1 +0.4 +0.8 N.D. N.D. N.D.
phage 36hr PHYTO4 MOI 1 +0.2 +0.9 N.D. N.D. N.D.
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