Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS12155 and BPHYT_RS12160 are separated by 128 nucleotidesBPHYT_RS12160 and BPHYT_RS12165 overlap by 4 nucleotidesBPHYT_RS12165 and BPHYT_RS12170 are separated by 190 nucleotidesBPHYT_RS12170 and BPHYT_RS12175 are separated by 116 nucleotides BPHYT_RS12155: BPHYT_RS12155 - RNA-binding protein S4, at 2,772,331 to 2,773,083 _RS12155 BPHYT_RS12160: BPHYT_RS12160 - peptide deformylase, at 2,773,212 to 2,773,745 _RS12160 BPHYT_RS12165: BPHYT_RS12165 - DNA ligase, at 2,773,742 to 2,775,808 _RS12165 BPHYT_RS12170: BPHYT_RS12170 - cell division protein FtsZ, at 2,775,999 to 2,777,288 _RS12170 BPHYT_RS12175: BPHYT_RS12175 - chromosome segregation protein SMC, at 2,777,405 to 2,780,923 _RS12175
Group Condition BPHYT_RS12155 BPHYT_RS12160 BPHYT_RS12165 BPHYT_RS12170 BPHYT_RS12175
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -1.8 -0.7 N.D. N.D. N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.7 -1.4 N.D. N.D. N.D.
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -0.1 -1.7 N.D. N.D. N.D.
phage 36hr PHYTO1 MOI 1 -1.1 -0.5 N.D. N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X -0.1 -1.5 N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_R2A_with_Tween_and_Cycloheximide; Time=15_days -0.5 -0.9 N.D. N.D. N.D.
nophagecontrol 36hr Phyto Lib only -0.2 -1.1 N.D. N.D. N.D.
phage 24hr PHYTO7 MOI 1 -0.6 -0.7 N.D. N.D. N.D.
supernatant control Vogels_fungal_media 0.1X -0.3 -0.9 N.D. N.D. N.D.
phage 24hr PHYTO3 MOI 10 -0.8 -0.3 N.D. N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X -0.5 -0.6 N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.7 +0.3 N.D. N.D. N.D.
carbon source L-Arabinose (C) +0.4 -0.7 N.D. N.D. N.D.
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X -0.8 +0.5 N.D. N.D. N.D.
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days -0.9 +0.6 N.D. N.D. N.D.
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 -0.8 +0.8 N.D. N.D. N.D.
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days -0.5 +0.5 N.D. N.D. N.D.
motility outer cut, LB soft agar motility assay -0.6 +0.8 N.D. N.D. N.D.
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -0.6 +0.9 N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.4 +0.7 N.D. N.D. N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -0.4 +0.7 N.D. N.D. N.D.
motility inner cut, LB soft agar motility assay -0.4 +0.8 N.D. N.D. N.D.
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent -0.3 +0.9 N.D. N.D. N.D.
stress Benzethonium chloride 0.0016 mM -0.1 +0.9 N.D. N.D. N.D.
nitrogen source L-Lysine (N) -0.5 +1.4 N.D. N.D. N.D.
temperature LB, 37C -0.3 +1.3 N.D. N.D. N.D.
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) +0.4 +0.7 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.1 +1.2 N.D. N.D. N.D.
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) +0.7 +0.7 N.D. N.D. N.D.
stress Bacitracin 0.25 mg/ml +0.1 +1.3 N.D. N.D. N.D.
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