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Protein
Homologs
Fitness for 5 genes in
Burkholderia phytofirmans PsJN
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
BPHYT_RS12155 and BPHYT_RS12160 are separated by 128 nucleotides
BPHYT_RS12160 and BPHYT_RS12165 overlap by 4 nucleotides
BPHYT_RS12165 and BPHYT_RS12170 are separated by 190 nucleotides
BPHYT_RS12170 and BPHYT_RS12175 are separated by 116 nucleotides
BPHYT_RS12155: BPHYT_RS12155 - RNA-binding protein S4, at 2,772,331 to 2,773,083
_RS12155
BPHYT_RS12160: BPHYT_RS12160 - peptide deformylase, at 2,773,212 to 2,773,745
_RS12160
BPHYT_RS12165: BPHYT_RS12165 - DNA ligase, at 2,773,742 to 2,775,808
_RS12165
BPHYT_RS12170: BPHYT_RS12170 - cell division protein FtsZ, at 2,775,999 to 2,777,288
_RS12170
BPHYT_RS12175: BPHYT_RS12175 - chromosome segregation protein SMC, at 2,777,405 to 2,780,923
_RS12175
Group
Condition
BPHYT
_RS12155
BPHYT
_RS12160
BPHYT
_RS12165
BPHYT
_RS12170
BPHYT
_RS12175
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB
-1.8
-0.7
N.D.
N.D.
N.D.
supernatant control
Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB
-0.7
-1.4
N.D.
N.D.
N.D.
in planta
Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days
-0.1
-1.7
N.D.
N.D.
N.D.
phage
36hr PHYTO1 MOI 1
-1.1
-0.5
N.D.
N.D.
N.D.
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X
-0.1
-1.5
N.D.
N.D.
N.D.
in planta
Plant=Zea_mays; PlantTreatment=None; sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_R2A_with_Tween_and_Cycloheximide; Time=15_days
-0.5
-0.9
N.D.
N.D.
N.D.
nophagecontrol
36hr Phyto Lib only
-0.2
-1.1
N.D.
N.D.
N.D.
phage
24hr PHYTO7 MOI 1
-0.6
-0.7
N.D.
N.D.
N.D.
supernatant control
Vogels_fungal_media 0.1X
-0.3
-0.9
N.D.
N.D.
N.D.
phage
24hr PHYTO3 MOI 10
-0.8
-0.3
N.D.
N.D.
N.D.
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X
-0.5
-0.6
N.D.
N.D.
N.D.
in planta
Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.7
+0.3
N.D.
N.D.
N.D.
carbon source
L-Arabinose (C)
+0.4
-0.7
N.D.
N.D.
N.D.
supernatant control:fungal media
R2A with Vogels_fungal_media 0.8X
-0.8
+0.5
N.D.
N.D.
N.D.
in planta
Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days
-0.9
+0.6
N.D.
N.D.
N.D.
tn7 efficiency
Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50
-0.8
+0.8
N.D.
N.D.
N.D.
in planta
Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days
-0.5
+0.5
N.D.
N.D.
N.D.
motility
outer cut, LB soft agar motility assay
-0.6
+0.8
N.D.
N.D.
N.D.
in planta
Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days
-0.6
+0.9
N.D.
N.D.
N.D.
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
-0.4
+0.7
N.D.
N.D.
N.D.
in planta
Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days
-0.4
+0.7
N.D.
N.D.
N.D.
motility
inner cut, LB soft agar motility assay
-0.4
+0.8
N.D.
N.D.
N.D.
lb_dap plate control and outgrow on gent
LB_DAP agar plate, outgrowth in LB_gent
-0.3
+0.9
N.D.
N.D.
N.D.
stress
Benzethonium chloride 0.0016 mM
-0.1
+0.9
N.D.
N.D.
N.D.
nitrogen source
L-Lysine (N)
-0.5
+1.4
N.D.
N.D.
N.D.
temperature
LB, 37C
-0.3
+1.3
N.D.
N.D.
N.D.
carbon source
2-Deoxyadenosine 5-monophosphate 5 mM (C)
+0.4
+0.7
N.D.
N.D.
N.D.
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
+0.1
+1.2
N.D.
N.D.
N.D.
carbon source
Deoxyribonucleic from herring sperm 10 mg/mL (C)
+0.7
+0.7
N.D.
N.D.
N.D.
stress
Bacitracin 0.25 mg/ml
+0.1
+1.3
N.D.
N.D.
N.D.
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