Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS11940 and BPHYT_RS11945 are separated by 111 nucleotidesBPHYT_RS11945 and BPHYT_RS11950 are separated by 208 nucleotidesBPHYT_RS11950 and BPHYT_RS11955 are separated by 104 nucleotidesBPHYT_RS11955 and BPHYT_RS11960 are separated by 34 nucleotides BPHYT_RS11940: BPHYT_RS11940 - hypothetical protein, at 2,715,706 to 2,716,509 _RS11940 BPHYT_RS11945: BPHYT_RS11945 - multidrug transporter, at 2,716,621 to 2,717,628 _RS11945 BPHYT_RS11950: BPHYT_RS11950 - DSBA oxidoreductase, at 2,717,837 to 2,719,390 _RS11950 BPHYT_RS11955: BPHYT_RS11955 - hemolysin D, at 2,719,495 to 2,720,808 _RS11955 BPHYT_RS11960: BPHYT_RS11960 - MarR family transcriptional regulator, at 2,720,843 to 2,722,360 _RS11960
Group Condition BPHYT_RS11940 BPHYT_RS11945 BPHYT_RS11950 BPHYT_RS11955 BPHYT_RS11960
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.5 +0.2 -0.7 -4.7
phage 24hr PHYTO11 MOI 0.1 N.D. +0.0 +0.1 -3.6 -1.5
phage 24hr PHYTO2 MOI 0.1 N.D. +0.2 -0.2 -1.8 -2.7
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -1.1 -0.5 -0.5 -2.2
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. -0.6 -0.4 -0.3 -3.0
phage P. phyto PHYTO2 MOI 1 N.D. +0.3 -1.0 -2.6 -0.7
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.8 +0.2 -0.5 -2.3
phage 36hr PHYTO9 MOI 1 N.D. +0.3 -0.6 -1.9 -1.2
phage 24hr PHYTO4 MOI 0.1 N.D. -0.4 -0.2 -3.5 +0.8
phage 24hr PHYTO9 MOI 0.1 N.D. -0.2 +0.1 +0.1 -3.2
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) N.D. -0.5 +0.1 -0.3 -2.5
phage 24hr PHYTO8 MOI 1 N.D. +0.1 +0.1 -0.2 -3.1
phage 24hr PHYTO3 MOI 1 N.D. +0.3 -0.2 -0.6 -2.7
phage 24hr PHYTO3 MOI 0.1 N.D. +0.2 -0.1 -2.0 -1.1
lb_dap plate control LB_DAP agar plate, outgrowth in LB N.D. -0.3 +0.3 -0.5 -2.2
phage 24hr PHYTO MOI 0.1 N.D. +0.6 -0.4 -0.3 -2.7
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.1 -0.4 +0.4 -2.6
phage 36hr PHYTO2 MOI 0.1 N.D. +0.2 -0.2 +0.3 -2.8
phage 24hr PHYTO7 MOI 10 N.D. +0.8 -0.5 -2.1 -0.6
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.4 -0.7 -1.1 -0.9
carbon source Protocatechuic Acid (C) N.D. +0.5 +0.0 -0.8 -1.9
phage 24hr PHYTO9 MOI 10 N.D. +0.1 +0.1 +0.7 -3.1
phage 36hr PHYTO8 MOI 10 N.D. +0.2 +0.6 -1.6 -1.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. +0.5 +0.4 -0.3 -2.0
phage 24hr PHYTO4 MOI 10 N.D. +0.1 +0.1 -2.4 +1.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB N.D. +1.2 +0.5 -1.0 -0.8
nophagecontrol 36hr Phyto Lib only N.D. +0.4 -0.8 -0.7 +1.6
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB N.D. +0.6 +0.7 -0.8 +1.4
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 N.D. +1.0 +0.7 +0.4 +1.6
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 N.D. -0.3 +0.6 +2.2 +1.4
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BPHYT_RS11940
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