Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS11810 and BPHYT_RS11815 are separated by 238 nucleotidesBPHYT_RS11815 and BPHYT_RS11820 are separated by 144 nucleotidesBPHYT_RS11820 and BPHYT_RS11825 are separated by 45 nucleotidesBPHYT_RS11825 and BPHYT_RS11830 overlap by 4 nucleotides BPHYT_RS11810: BPHYT_RS11810 - LysR family transcriptional regulator, at 2,677,721 to 2,678,599 _RS11810 BPHYT_RS11815: BPHYT_RS11815 - alcohol dehydrogenase, at 2,678,838 to 2,679,863 _RS11815 BPHYT_RS11820: BPHYT_RS11820 - amidohydrolase, at 2,680,008 to 2,681,459 _RS11820 BPHYT_RS11825: BPHYT_RS11825 - cytosine deaminase, at 2,681,505 to 2,682,710 _RS11825 BPHYT_RS11830: BPHYT_RS11830 - sodium:solute symporter, at 2,682,707 to 2,684,185 _RS11830
Group Condition BPHYT_RS11810 BPHYT_RS11815 BPHYT_RS11820 BPHYT_RS11825 BPHYT_RS11830
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 -0.2 -0.5 -5.4 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -1.0 -0.1 +0.4 -5.0 +0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.4 -0.3 -0.5 -1.6 -0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB -3.4 -0.7 +1.3 -1.5 +0.6
phage 36hr PHYTO9 MOI 1 -0.7 +0.7 -0.2 -3.1 +0.0
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.3 -0.3 -0.4 -1.4 +0.4
phage 36hr PHYTO9 MOI 0.1 -0.2 +1.1 -0.4 -3.1 -0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +1.0 +0.9 +0.1 -4.9 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.2 -0.5 +0.7 -2.6 +0.1
phage 24hr PHYTO6 MOI 10 -2.0 +1.2 -0.3 -1.5 +0.1
phage 24hr PHYTO9 MOI 10 +0.4 +1.2 -0.5 -3.5 -0.0
phage 24hr PHYTO MOI 0.1 -0.1 +1.0 +0.0 -3.0 -0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days +0.2 +0.3 +0.1 -3.1 +0.5
phage 24hr PHYTO2 MOI 0.1 +0.2 +0.7 +0.2 -3.0 -0.1
phage 24hr PHYTO10 MOI 10 +0.3 +0.3 +0.6 -3.0 +0.0
phage 36hr PHYTO11 MOI 0.1 +0.6 +1.0 +0.2 -3.0 +0.1
carbon source Malonic (C) -1.1 +0.9 -0.6 -0.7 +0.5
nophagecontrol 36hr Phyto Lib only -1.3 +1.3 -0.3 -0.6 -0.1
phage 24hr PHYTO6 MOI 1 +0.1 +1.2 +0.5 -3.0 +0.3
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -1.4 +0.5 -0.7 +0.9 +0.0
phage PHYTO3 MOI 0.1 +1.2 -2.5 +0.4 +0.3 +0.4
phage P. phyto corn MOI 0.1 +1.5 +0.3 +0.4 -2.1 -0.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +2.7 -0.3 -0.3 -0.8 +0.0
phage P. phyto corn MOI 1 -1.2 +1.1 +0.7 +0.6 +0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +3.5 -0.5 -0.4 -0.9 +0.1
phage 36hr PHYTO7 MOI 1 -0.6 +1.6 -0.2 +1.1 -0.1
supernatant control Vogels_fungal_media; pH 5.5 +0.4 +1.0 +1.3 -0.9 +0.4
phage 36hr PHYTO4 MOI 1 +0.6 +1.9 +0.8 +0.4 +0.3
supernatant control Vogels_fungal_media 0.1X +1.5 +0.4 +0.8 +0.9 +0.4
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +3.6 +0.5 -0.4 +1.4 +0.1
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BPHYT_RS11810
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BPHYT_RS11815
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BPHYT_RS11825
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BPHYT_RS11830
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