Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS11520 and BPHYT_RS11525 are separated by 13 nucleotidesBPHYT_RS11525 and BPHYT_RS11530 are separated by 340 nucleotidesBPHYT_RS11530 and BPHYT_RS11535 are separated by 338 nucleotidesBPHYT_RS11535 and BPHYT_RS11540 are separated by 435 nucleotides BPHYT_RS11520: BPHYT_RS11520 - hydroxydechloroatrazine ethylaminohydrolase, at 2,620,043 to 2,621,440 _RS11520 BPHYT_RS11525: BPHYT_RS11525 - hypothetical protein, at 2,621,454 to 2,621,636 _RS11525 BPHYT_RS11530: BPHYT_RS11530 - 5-hydroxyisourate hydrolase, at 2,621,977 to 2,622,330 _RS11530 BPHYT_RS11535: BPHYT_RS11535 - membrane protein, at 2,622,669 to 2,623,862 _RS11535 BPHYT_RS11540: BPHYT_RS11540 - ureidoglycolate hydrolase, at 2,624,298 to 2,624,810 _RS11540
Group Condition BPHYT_RS11520 BPHYT_RS11525 BPHYT_RS11530 BPHYT_RS11535 BPHYT_RS11540
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.5 N.D. -0.6 -2.9 -1.2
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) -0.3 N.D. -0.8 -1.7 -1.5
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) -0.2 N.D. -2.2 -1.0 -0.3
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) +0.0 N.D. -0.7 -1.2 -1.0
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent -0.3 N.D. -0.8 -1.6 -0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.3 N.D. -0.7 -0.7 -0.9
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.0 N.D. -0.4 -1.1 -1.1
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent +0.3 N.D. +0.4 -0.9 -2.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.4 N.D. -0.9 -0.2 -1.4
phage 36hr PHYTO3 MOI 1 -0.1 N.D. -0.4 +0.4 -2.1
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.3 N.D. -0.8 -1.1 -0.5
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days +0.3 N.D. -0.3 +0.7 -2.7
phage 24hr PHYTO2 MOI 1 -0.2 N.D. +0.1 +0.2 -2.0
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days +0.1 N.D. +0.1 +0.5 -2.5
nitrogen source L-Lysine (N) +0.3 N.D. +0.3 -1.4 -0.9
phage 24hr PHYTO3 MOI 10 +0.4 N.D. -0.9 -0.7 -0.5
phage 36hr PHYTO2 MOI 1 -0.3 N.D. -2.0 +1.1 -0.4
phage 36hr PHYTO1 MOI 10 -0.1 N.D. -1.0 +0.5 -1.0
motility inner cut, LB soft agar motility assay +0.2 N.D. -0.5 -1.5 +0.3
phage P. phyto PHYTO2 MOI 1 -0.3 N.D. +0.0 +0.6 -1.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.0 N.D. -0.9 +1.2 -1.3
vitamin With vitamins t2 +0.1 N.D. -0.6 -1.4 +1.0
phage 24hr PHYTO6 MOI 10 -0.3 N.D. -1.0 +0.4 +0.8
carbon source Suberic (C) +0.3 N.D. -0.6 -0.9 +1.1
phage 24hr PHYTO7 MOI 1 +0.1 N.D. -1.2 +0.8 +0.7
carbon source Malonic (C) -0.2 N.D. -0.6 -0.6 +1.7
phage 36hr PHYTO2 MOI 1 +0.3 N.D. -1.0 +0.8 +0.4
vitamin With vitamins t1 +0.2 N.D. +0.4 -1.0 +1.0
phage 24hr PHYTO9 MOI 10 +0.1 N.D. -0.6 +1.1 +0.7
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +0.1 N.D. +1.9 -0.1 +1.5
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