Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS11490 and BPHYT_RS11495 are separated by 133 nucleotidesBPHYT_RS11495 and BPHYT_RS11500 are separated by 53 nucleotidesBPHYT_RS11500 and BPHYT_RS11505 are separated by 44 nucleotidesBPHYT_RS11505 and BPHYT_RS11510 are separated by 75 nucleotides BPHYT_RS11490: BPHYT_RS11490 - hypothetical protein, at 2,613,548 to 2,613,754 _RS11490 BPHYT_RS11495: BPHYT_RS11495 - membrane protein, at 2,613,888 to 2,614,994 _RS11495 BPHYT_RS11500: BPHYT_RS11500 - ABC transporter permease, at 2,615,048 to 2,615,977 _RS11500 BPHYT_RS11505: BPHYT_RS11505 - sugar ABC transporter permease, at 2,616,022 to 2,617,128 _RS11505 BPHYT_RS11510: BPHYT_RS11510 - ABC transporter, at 2,617,204 to 2,618,805 _RS11510
Group Condition BPHYT_RS11490 BPHYT_RS11495 BPHYT_RS11500 BPHYT_RS11505 BPHYT_RS11510
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. -0.6 -0.2 -0.6 -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. -0.2 +0.1 -0.9 -0.7
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.1 +0.0 -1.0 -0.9
supernatant control Vogels_fungal_media; pH 5.5 N.D. -0.3 -0.7 -0.8 +0.2
phage 24hr PHYTO9 MOI 10 N.D. +0.6 +0.3 -1.1 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. -0.8 +0.8 +0.0 -0.4
carbon source adipate (C) N.D. -0.5 +0.7 +0.1 -0.6
nitrogen source Putrescine (N) N.D. +0.5 -0.7 -0.4 +0.5
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. -0.3 +1.2 -0.4 -0.5
phage 24hr PHYTO11 MOI 1 N.D. +0.3 +0.8 -0.3 -0.8
phage 36hr PHYTO MOI 0.1 N.D. +1.1 -0.3 -0.5 -0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. -0.0 +1.1 -0.4 -0.3
carbon source Protocatechuic Acid (C) N.D. +0.2 +0.8 -0.7 +0.2
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days N.D. +1.3 +0.3 -0.1 -0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. -0.6 +0.1 +0.4 +0.8
carbon source Sebacic (C) N.D. +0.2 +1.2 -0.0 -0.6
nophagecontrol 36hr Phyto Lib only N.D. +0.9 +0.6 -0.2 -0.4
phage 24hr PHYTO3 MOI 1 N.D. +1.0 +0.2 -0.5 +0.4
nophagecontrol 36hr Phyto Lib only N.D. +1.1 +0.5 -0.4 -0.2
nophagecontrol 24hr Phyto Lib Only N.D. +0.6 -0.4 +0.4 +0.4
phage 24hr PHYTO2 MOI 1 N.D. +0.8 +0.8 -0.1 -0.5
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days N.D. +0.3 +0.4 +1.0 -0.6
phage 36hr PHYTO4 MOI 1 N.D. +0.9 +0.8 +0.3 -0.6
phage 24hr PHYTO3 MOI 10 N.D. +0.6 -0.2 +0.2 +0.8
motility inner cut, LB soft agar motility assay N.D. +0.5 +1.0 -0.2 +0.1
phage 36hr PHYTO6 MOI 0.1 N.D. +0.6 +0.5 -0.2 +0.6
phage 24hr PHYTO3 MOI 10 N.D. +0.3 -0.2 +0.6 +0.9
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days N.D. +0.3 +0.9 +0.1 +0.5
lb_dap plate control LB_DAP agar plate, outgrowth in LB N.D. +0.7 +0.7 +0.4 +0.1
phage 36hr PHYTO2 MOI 1 N.D. +0.9 +0.9 -0.5 +0.7
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