Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS11110 and BPHYT_RS11115 are separated by 191 nucleotidesBPHYT_RS11115 and BPHYT_RS11120 are separated by 82 nucleotidesBPHYT_RS11120 and BPHYT_RS11125 are separated by 239 nucleotidesBPHYT_RS11125 and BPHYT_RS11130 are separated by 36 nucleotides BPHYT_RS11110: BPHYT_RS11110 - chemotaxis protein CheY, at 2,524,442 to 2,526,415 _RS11110 BPHYT_RS11115: BPHYT_RS11115 - hypothetical protein, at 2,526,607 to 2,526,849 _RS11115 BPHYT_RS11120: BPHYT_RS11120 - ferredoxin--NADP reductase, at 2,526,932 to 2,527,981 _RS11120 BPHYT_RS11125: BPHYT_RS11125 - alkylhydroperoxidase, at 2,528,221 to 2,528,658 _RS11125 BPHYT_RS11130: BPHYT_RS11130 - chemotaxis protein, at 2,528,695 to 2,530,269 _RS11130
Group Condition BPHYT_RS11110 BPHYT_RS11115 BPHYT_RS11120 BPHYT_RS11125 BPHYT_RS11130
phage PHYTO3 MOI 0.1 -0.3 N.D. N.D. -4.1 -0.3
phage 24hr PHYTO6 MOI 0.1 -0.5 N.D. N.D. -3.8 -0.2
phage 24hr PHYTO11 MOI 0.1 -0.5 N.D. N.D. -3.8 +0.1
phage 36hr PHYTO8 MOI 0.1 -0.0 N.D. N.D. -3.6 -0.3
phage P. phyto PHYTO2 MOI 1 -0.6 N.D. N.D. -1.2 -2.0
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.1 N.D. N.D. -3.0 -0.6
phage 24hr PHYTO3 MOI 10 +0.5 N.D. N.D. -4.0 -0.1
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.0 N.D. N.D. -3.1 -0.4
phage P. phyto PHYTO2 MOI 0.1 -0.1 N.D. N.D. -3.6 +0.3
phage 24hr PHYTO3 MOI 10 +0.2 N.D. N.D. -3.9 +0.4
phage 24hr PHYTO6 MOI 10 +0.1 N.D. N.D. -3.5 +0.2
phage 36hr PHYTO6 MOI 0.1 -0.6 N.D. N.D. -2.2 -0.2
phage 36hr PHYTO4 MOI 1 -0.7 N.D. N.D. -2.0 -0.3
phage 24hr PHYTO9 MOI 10 -0.8 N.D. N.D. -2.3 +0.1
phage 36hr PHYTO3 MOI 0.1 -0.5 N.D. N.D. -1.6 -0.8
phage 36hr PHYTO3 MOI 1 -1.4 N.D. N.D. -1.6 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.3 N.D. N.D. -1.8 -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.7 N.D. N.D. -1.5 -0.4
phage P. phyto PHYTO2 MOI 1 -0.4 N.D. N.D. -0.6 -1.4
phage 36hr PHYTO1 MOI 1 -0.4 N.D. N.D. -2.3 +0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 N.D. N.D. -2.7 +0.5
motility inner cut, LB soft agar motility assay -0.7 N.D. N.D. -1.4 +0.2
nophagecontrol 36hr Phyto Lib only +0.4 N.D. N.D. -2.0 +0.4
phage 24hr PHYTO4 MOI 10 -1.5 N.D. N.D. +0.6 -0.3
phage 24hr PHYTO7 MOI 1 -1.1 N.D. N.D. +1.2 -0.2
phage P. phyto corn MOI 1 +0.4 N.D. N.D. +0.9 -0.9
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 +1.5 N.D. N.D. -1.1 +0.2
motility outer cut, LB soft agar motility assay -0.5 N.D. N.D. +2.0 -0.5
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days -0.2 N.D. N.D. +2.7 -0.0
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +0.2 N.D. N.D. +3.0 -0.2
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