Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS11100 and BPHYT_RS11105 are separated by 24 nucleotidesBPHYT_RS11105 and BPHYT_RS11110 are separated by 197 nucleotidesBPHYT_RS11110 and BPHYT_RS11115 are separated by 191 nucleotidesBPHYT_RS11115 and BPHYT_RS11120 are separated by 82 nucleotides BPHYT_RS11100: BPHYT_RS11100 - methyltransferase, at 2,522,436 to 2,523,041 _RS11100 BPHYT_RS11105: BPHYT_RS11105 - acyl-CoA dehydrogenase, at 2,523,066 to 2,524,244 _RS11105 BPHYT_RS11110: BPHYT_RS11110 - chemotaxis protein CheY, at 2,524,442 to 2,526,415 _RS11110 BPHYT_RS11115: BPHYT_RS11115 - hypothetical protein, at 2,526,607 to 2,526,849 _RS11115 BPHYT_RS11120: BPHYT_RS11120 - ferredoxin--NADP reductase, at 2,526,932 to 2,527,981 _RS11120
Group Condition BPHYT_RS11100 BPHYT_RS11105 BPHYT_RS11110 BPHYT_RS11115 BPHYT_RS11120
motility outer cut, LB soft agar motility assay -0.2 -0.6 -1.2 N.D. N.D.
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 -1.2 -0.5 -0.3 N.D. N.D.
nophagecontrol 36hr Phyto Lib only -0.3 -0.5 -1.1 N.D. N.D.
phage P. phyto PHYTO2 MOI 1 -0.7 -0.5 -0.4 N.D. N.D.
phage 36hr PHYTO7 MOI 1 -1.1 -0.3 -0.1 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.4 -0.6 -0.4 N.D. N.D.
phage 24hr PHYTO4 MOI 10 +0.2 -0.2 -1.5 N.D. N.D.
nitrogen source Putrescine (N) -1.4 +0.1 -0.0 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 -0.3 -1.2 N.D. N.D.
phage 24hr PHYTO7 MOI 1 +0.2 -0.3 -1.1 N.D. N.D.
phage 24hr PHYTO4 MOI 0.1 +0.5 -0.4 -1.2 N.D. N.D.
phage 36hr PHYTO6 MOI 10 +0.2 -0.5 -0.8 N.D. N.D.
phage 36hr PHYTO3 MOI 1 +0.4 -0.1 -1.4 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 -1.0 +0.3 N.D. N.D.
phage 24hr PHYTO6 MOI 0.1 +0.4 +0.2 -1.3 N.D. N.D.
phage 24hr PHYTO11 MOI 1 +0.5 -0.2 -0.8 N.D. N.D.
phage 36hr PHYTO4 MOI 1 +0.5 -0.3 -0.7 N.D. N.D.
phage 24hr PHYTO MOI 0.1 +0.5 +0.1 -1.0 N.D. N.D.
phage 24hr PHYTO6 MOI 1 +0.6 +0.1 -0.8 N.D. N.D.
phage 36hr PHYTO8 MOI 10 +0.5 +0.3 -0.8 N.D. N.D.
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 +0.5 +0.2 -0.7 N.D. N.D.
carbon source D-Glucose (C) +0.9 +0.1 -0.9 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.8 -0.6 +0.2 N.D. N.D.
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days +0.8 +0.3 -0.6 N.D. N.D.
phage 24hr PHYTO10 MOI 10 +1.0 -0.3 -0.2 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +1.3 -0.5 -0.3 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.3 +0.9 -0.3 N.D. N.D.
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days +1.0 +0.4 -0.3 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +1.3 +0.1 -0.3 N.D. N.D.
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 -0.4 +0.0 +1.5 N.D. N.D.
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