Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS11085 and BPHYT_RS11090 are separated by 10 nucleotidesBPHYT_RS11090 and BPHYT_RS11095 are separated by 35 nucleotidesBPHYT_RS11095 and BPHYT_RS11100 overlap by 4 nucleotidesBPHYT_RS11100 and BPHYT_RS11105 are separated by 24 nucleotides BPHYT_RS11085: BPHYT_RS11085 - major facilitator transporter, at 2,519,848 to 2,521,245 _RS11085 BPHYT_RS11090: BPHYT_RS11090 - membrane protein, at 2,521,256 to 2,521,747 _RS11090 BPHYT_RS11095: BPHYT_RS11095 - glycosyl transferase, at 2,521,783 to 2,522,439 _RS11095 BPHYT_RS11100: BPHYT_RS11100 - methyltransferase, at 2,522,436 to 2,523,041 _RS11100 BPHYT_RS11105: BPHYT_RS11105 - acyl-CoA dehydrogenase, at 2,523,066 to 2,524,244 _RS11105
Group Condition BPHYT_RS11085 BPHYT_RS11090 BPHYT_RS11095 BPHYT_RS11100 BPHYT_RS11105
phage P. phyto PHYTO2 MOI 1 -0.1 N.D. -0.5 -0.7 -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 N.D. -0.4 -0.2 -1.0
phage 36hr PHYTO7 MOI 1 +0.2 N.D. -0.4 -1.1 -0.3
phage 36hr PHYTO9 MOI 0.1 -0.2 N.D. -1.5 +0.0 +0.1
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 -0.2 N.D. +0.4 -1.2 -0.5
phage 24hr PHYTO6 MOI 10 +0.1 N.D. -0.3 -0.2 -1.0
phage 24hr PHYTO7 MOI 10 +0.3 N.D. -1.2 +0.3 -0.6
nitrogen source Putrescine (N) -0.2 N.D. +0.3 -1.4 +0.1
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.5 N.D. -0.8 -0.3 -0.4
nitrogen source L-Isoleucine (N) -0.3 N.D. +0.5 +0.2 -1.1
nophagecontrol 24hr Phyto Lib only -0.0 N.D. -0.5 +0.5 -0.7
motility outer cut, LB soft agar motility assay -0.3 N.D. +0.5 -0.2 -0.6
carbon source 2-Deoxy-D-ribonic lithium salt (C) -0.3 N.D. -0.5 +0.9 -0.3
lb_nosalt LB_noSalt -0.3 N.D. -0.8 +0.9 +0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.5 N.D. +0.7 -0.5 -0.7
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days +0.2 N.D. -0.4 -0.4 +0.6
nitrogen source L-Serine (N) -0.3 N.D. +0.9 -0.3 -0.3
motility inner cut, LB soft agar motility assay -0.3 N.D. +0.8 -0.4 -0.0
carbon source D-Glucose (C) -0.1 N.D. -0.5 +0.9 +0.1
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -0.1 N.D. -0.5 +0.6 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.1 N.D. -0.2 +1.3 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.1 N.D. -0.6 +0.3 +0.9
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.8 N.D. +0.0 -0.5 +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 N.D. +0.4 +0.8 -0.6
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.0 N.D. -0.3 +1.3 +0.1
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.7 N.D. -0.3 +0.2 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.2 N.D. -0.2 +0.9 +0.4
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -0.2 N.D. +0.1 +1.0 +0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.4 N.D. +0.2 +1.0 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.2 N.D. +1.9 +0.3 -0.1
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