Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS10815 and BPHYT_RS10820 are separated by 3 nucleotidesBPHYT_RS10820 and BPHYT_RS10825 overlap by 8 nucleotidesBPHYT_RS10825 and BPHYT_RS10830 are separated by 23 nucleotidesBPHYT_RS10830 and BPHYT_RS10835 overlap by 4 nucleotides BPHYT_RS10815: BPHYT_RS10815 - crotonase, at 2,458,834 to 2,459,640 _RS10815 BPHYT_RS10820: BPHYT_RS10820 - 4-chlorobenzoate--CoA ligase, at 2,459,644 to 2,461,221 _RS10820 BPHYT_RS10825: BPHYT_RS10825 - (2Fe-2S)-binding protein, at 2,461,214 to 2,461,744 _RS10825 BPHYT_RS10830: BPHYT_RS10830 - carbon monoxide dehydrogenase, at 2,461,768 to 2,462,589 _RS10830 BPHYT_RS10835: BPHYT_RS10835 - carbon monoxide dehydrogenase, at 2,462,586 to 2,465,624 _RS10835
Group Condition BPHYT_RS10815 BPHYT_RS10820 BPHYT_RS10825 BPHYT_RS10830 BPHYT_RS10835
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. -0.6 N.D. -1.4 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. +0.1 N.D. -2.0 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.0 N.D. -1.6 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. +0.0 N.D. -1.6 -0.3
in planta Plant=Brachypodium_distachyon ; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -0.2 N.D. -1.3 -0.3
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days N.D. -0.2 N.D. -1.2 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. -0.4 N.D. -1.0 -0.1
carbon source D-Glucose (C) N.D. -0.3 N.D. -0.8 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. +0.0 N.D. -1.2 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. +0.2 N.D. -1.2 -0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.2 N.D. -1.1 -0.1
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days N.D. +0.2 N.D. -0.8 -0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB N.D. +0.3 N.D. -1.6 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.5 N.D. -0.8 -0.5
supernatant control Vogels_fungal_media; pH 5.5 N.D. +0.1 N.D. -1.1 +0.4
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. -0.4 N.D. -1.0 +0.7
phage 24hr PHYTO3 MOI 1 N.D. -0.8 N.D. +0.5 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.1 N.D. -0.9 +0.5
phage 36hr PHYTO4 MOI 1 N.D. +0.1 N.D. +0.6 -0.7
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.2 N.D. +0.9 -0.5
phage 36hr PHYTO3 MOI 1 N.D. -0.6 N.D. +1.1 -0.1
phage 36hr PHYTO8 MOI 0.1 N.D. -0.4 N.D. +0.9 -0.2
vitamin No vitamins t2 N.D. -0.4 N.D. +1.0 -0.1
phage P. phyto PHYTO2 MOI 1 N.D. +0.6 N.D. +0.3 -0.5
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days N.D. +0.3 N.D. -0.6 +0.8
phage 24hr PHYTO4 MOI 10 N.D. -0.1 N.D. +1.4 -0.5
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) N.D. -0.3 N.D. +0.7 +0.3
phage 24hr PHYTO MOI 0.1 N.D. -0.4 N.D. +1.1 +0.2
carbon source Azelaic (C) N.D. -0.5 N.D. +1.1 +0.4
phage 24hr PHYTO6 MOI 10 N.D. +0.5 N.D. +0.8 +0.3
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