Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS10745 and BPHYT_RS10750 are separated by 28 nucleotidesBPHYT_RS10750 and BPHYT_RS10755 are separated by 119 nucleotidesBPHYT_RS10755 and BPHYT_RS10760 overlap by 2 nucleotidesBPHYT_RS10760 and BPHYT_RS10765 are separated by 45 nucleotides BPHYT_RS10745: BPHYT_RS10745 - FMN reductase, at 2,444,072 to 2,444,611 _RS10745 BPHYT_RS10750: BPHYT_RS10750 - LysR family transcriptional regulator, at 2,444,640 to 2,445,572 _RS10750 BPHYT_RS10755: BPHYT_RS10755 - hypothetical protein, at 2,445,692 to 2,445,892 _RS10755 BPHYT_RS10760: BPHYT_RS10760 - major facilitator transporter, at 2,445,891 to 2,447,213 _RS10760 BPHYT_RS10765: BPHYT_RS10765 - amidase, at 2,447,259 to 2,448,722 _RS10765
Group Condition BPHYT_RS10745 BPHYT_RS10750 BPHYT_RS10755 BPHYT_RS10760 BPHYT_RS10765
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. -4.7 N.D. -0.2 -0.4
phage P. phyto PHYTO2 MOI 0.1 N.D. -3.6 N.D. -0.4 -0.8
phage P. phyto PHYTO2 MOI 1 N.D. -3.8 N.D. +0.9 -1.8
nophagecontrol 36hr Phyto Lib only N.D. -2.7 N.D. -0.6 -1.2
phage P. phyto PHYTO2 MOI 1 N.D. -1.3 N.D. -2.0 -1.1
phage 36hr PHYTO11 MOI 1 N.D. -2.8 N.D. -0.6 -0.9
phage 36hr PHYTO6 MOI 1 N.D. -2.6 N.D. -0.8 -0.8
nophagecontrol 36hr Phyto Lib only N.D. -2.7 N.D. -0.4 -1.1
phage 24hr PHYTO2 MOI 0.1 N.D. -2.7 N.D. +0.0 -1.3
carbon source adipate (C) N.D. -3.5 N.D. +0.2 -0.4
phage 24hr PHYTO3 MOI 10 N.D. -2.9 N.D. -0.8 +0.0
phage 24hr PHYTO6 MOI 10 N.D. -1.2 N.D. -0.2 -2.1
phage 36hr PHYTO2 MOI 1 N.D. -2.7 N.D. -1.0 +0.4
phage 36hr PHYTO2 MOI 1 N.D. -1.0 N.D. -1.0 -1.2
nophagecontrol 36hr Phyto Lib Only N.D. -2.9 N.D. +0.2 -0.4
phage 24hr PHYTO MOI 0.1 N.D. -2.7 N.D. -0.7 +0.3
phage 24hr PHYTO6 MOI 0.1 N.D. -1.2 N.D. -0.5 -1.3
phage 36hr PHYTO11 MOI 1 N.D. -1.2 N.D. -0.3 -1.4
phage 36hr PHYTO8 MOI 0.1 N.D. +0.6 N.D. -0.5 -3.0
phage 36hr PHYTO6 MOI 0.1 N.D. -2.6 N.D. +0.0 +0.1
phage 36hr PHYTO8 MOI 10 N.D. -2.6 N.D. -0.3 +0.5
phage 36hr PHYTO4 MOI 1 N.D. -2.4 N.D. -0.2 +0.5
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days N.D. -2.2 N.D. -0.1 +0.4
phage 36hr PHYTO3 MOI 1 N.D. -1.3 N.D. -1.1 +0.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -1.6 N.D. +0.9 -0.3
phage 36hr PHYTO10 MOI 10 N.D. +1.6 N.D. -1.3 -0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -1.1 N.D. +1.4 -0.4
phage 36hr PHYTO1 MOI 10 N.D. +1.9 N.D. +0.5 -2.1
phage 24hr PHYTO7 MOI 1 N.D. +1.6 N.D. +0.3 -1.3
vitamin With vitamins t1 N.D. +1.8 N.D. +0.6 -0.3
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