Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS10735 and BPHYT_RS10740 are separated by 51 nucleotidesBPHYT_RS10740 and BPHYT_RS10745 are separated by 258 nucleotidesBPHYT_RS10745 and BPHYT_RS10750 are separated by 28 nucleotidesBPHYT_RS10750 and BPHYT_RS10755 are separated by 119 nucleotides BPHYT_RS10735: BPHYT_RS10735 - 3-oxoacyl-ACP reductase, at 2,442,356 to 2,443,099 _RS10735 BPHYT_RS10740: BPHYT_RS10740 - LuxR family transcriptional regulator, at 2,443,151 to 2,443,813 _RS10740 BPHYT_RS10745: BPHYT_RS10745 - FMN reductase, at 2,444,072 to 2,444,611 _RS10745 BPHYT_RS10750: BPHYT_RS10750 - LysR family transcriptional regulator, at 2,444,640 to 2,445,572 _RS10750 BPHYT_RS10755: BPHYT_RS10755 - hypothetical protein, at 2,445,692 to 2,445,892 _RS10755
Group Condition BPHYT_RS10735 BPHYT_RS10740 BPHYT_RS10745 BPHYT_RS10750 BPHYT_RS10755
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. -3.1 N.D. -4.7 N.D.
phage 24hr PHYTO3 MOI 10 N.D. -3.5 N.D. -2.9 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. -5.1 N.D. -0.8 N.D.
phage P. phyto PHYTO2 MOI 1 N.D. -1.8 N.D. -3.8 N.D.
phage P. phyto PHYTO2 MOI 0.1 N.D. -1.6 N.D. -3.6 N.D.
phage 36hr PHYTO11 MOI 1 N.D. -1.8 N.D. -2.8 N.D.
nophagecontrol 36hr Phyto Lib only N.D. -0.8 N.D. -2.7 N.D.
phage 36hr PHYTO8 MOI 10 N.D. -0.8 N.D. -2.6 N.D.
carbon source adipate (C) N.D. +0.1 N.D. -3.5 N.D.
phage 36hr PHYTO4 MOI 1 N.D. -0.8 N.D. -2.4 N.D.
phage 24hr PHYTO4 MOI 10 N.D. -2.6 N.D. -0.6 N.D.
phage 24hr PHYTO9 MOI 10 N.D. -3.6 N.D. +0.7 N.D.
phage 36hr PHYTO6 MOI 1 N.D. -0.2 N.D. -2.6 N.D.
phage 24hr PHYTO MOI 0.1 N.D. -0.1 N.D. -2.7 N.D.
phage 24hr PHYTO2 MOI 0.1 N.D. +0.1 N.D. -2.7 N.D.
phage 36hr PHYTO2 MOI 1 N.D. +0.1 N.D. -2.7 N.D.
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days N.D. -2.0 N.D. -0.6 N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. -1.7 N.D. -0.8 N.D.
phage 36hr PHYTO2 MOI 1 N.D. -1.5 N.D. -1.0 N.D.
nophagecontrol 36hr Phyto Lib Only N.D. +0.5 N.D. -2.9 N.D.
nophagecontrol 36hr Phyto Lib only N.D. +0.3 N.D. -2.7 N.D.
nophagecontrol 24hr Phyto Lib only N.D. -2.2 N.D. +0.4 N.D.
phage 36hr PHYTO6 MOI 0.1 N.D. +0.9 N.D. -2.6 N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days N.D. +0.9 N.D. -2.2 N.D.
phage 24hr PHYTO10 MOI 10 N.D. -1.8 N.D. +0.6 N.D.
phage 36hr PHYTO2 MOI 0.1 N.D. -1.8 N.D. +0.8 N.D.
nitrogen source L-Phenylalanine (N) N.D. +0.8 N.D. -1.5 N.D.
phage 24hr PHYTO7 MOI 1 N.D. -0.8 N.D. +1.6 N.D.
phage 24hr PHYTO10 MOI 10 N.D. +1.9 N.D. -0.4 N.D.
phage 36hr PHYTO1 MOI 10 N.D. +1.6 N.D. +1.9 N.D.
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