Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS10695 and BPHYT_RS10700 are separated by 32 nucleotidesBPHYT_RS10700 and BPHYT_RS10705 are separated by 40 nucleotidesBPHYT_RS10705 and BPHYT_RS10710 are separated by 10 nucleotidesBPHYT_RS10710 and BPHYT_RS10715 are separated by 236 nucleotides BPHYT_RS10695: BPHYT_RS10695 - aromatic-ring-hydroxylating dioxygenase subunit beta, at 2,434,972 to 2,435,472 _RS10695 BPHYT_RS10700: BPHYT_RS10700 - 4-oxalocrotonate tautomerase, at 2,435,505 to 2,435,888 _RS10700 BPHYT_RS10705: BPHYT_RS10705 - MFS transporter, at 2,435,929 to 2,437,245 _RS10705 BPHYT_RS10710: BPHYT_RS10710 - sensor histidine kinase, at 2,437,256 to 2,438,776 _RS10710 BPHYT_RS10715: BPHYT_RS10715 - acyl-CoA dehydrogenase, at 2,439,013 to 2,440,191 _RS10715
Group Condition BPHYT_RS10695 BPHYT_RS10700 BPHYT_RS10705 BPHYT_RS10710 BPHYT_RS10715
phage 36hr PHYTO4 MOI 0.1 -1.4 N.D. -1.1 -1.7 +0.5
phage 36hr PHYTO10 MOI 10 -2.4 N.D. -0.5 -1.1 +0.3
phage 24hr PHYTO2 MOI 0.1 -2.6 N.D. -0.6 +0.0 -0.2
phage 36hr PHYTO6 MOI 0.1 -1.5 N.D. -0.5 -1.8 +0.7
phage 36hr PHYTO9 MOI 1 -0.3 N.D. -0.6 -2.3 +0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.9 N.D. +0.1 -1.4 -0.7
phage 24hr PHYTO4 MOI 0.1 -0.5 N.D. -1.4 -1.4 +0.4
phage 24hr PHYTO8 MOI 1 -0.8 N.D. -1.0 -1.5 +0.4
phage 36hr PHYTO8 MOI 0.1 -1.2 N.D. -0.2 -1.1 -0.2
phage 24hr PHYTO1 MOI 1 -2.0 N.D. +0.2 -0.7 +0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -1.1 N.D. +0.1 -1.2 -0.3
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days +0.3 N.D. +0.4 -1.5 -1.2
phage 36hr PHYTO8 MOI 10 -0.9 N.D. +0.2 -1.6 +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.6 N.D. +0.1 -1.4 +0.9
lb_dap plate control LB_DAP agar plate, outgrowth in LB +0.6 N.D. -0.8 -1.3 -0.4
phage P. phyto PHYTO2 MOI 1 +0.3 N.D. +0.4 -2.4 -0.1
phage 36hr PHYTO3 MOI 1 +0.4 N.D. -1.9 -0.2 +0.3
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 -1.2 N.D. -0.9 +0.7 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.6 N.D. -0.7 -1.3 +0.2
phage 36hr PHYTO6 MOI 0.1 -1.9 N.D. -0.0 +0.5 +0.2
phage 24hr PHYTO9 MOI 10 -0.3 N.D. +0.8 -0.9 -0.7
phage 24hr PHYTO11 MOI 0.1 -1.6 N.D. -0.2 +0.9 -0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.4 N.D. +0.6 -1.2 -0.6
phage 36hr PHYTO2 MOI 1 -0.2 N.D. -1.4 +0.7 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.6 N.D. +0.4 -1.7 +0.3
motility inner cut, LB soft agar motility assay +1.3 N.D. -0.6 -1.1 +0.1
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -1.3 N.D. -0.5 +0.6 +1.1
carbon source Suberic (C) +0.8 N.D. -1.5 +0.1 +0.7
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -1.2 N.D. +0.9 +0.9 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +2.1 N.D. -0.8 -0.9 +0.1
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