Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS10665 and BPHYT_RS10670 are separated by 140 nucleotidesBPHYT_RS10670 and BPHYT_RS10675 are separated by 69 nucleotidesBPHYT_RS10675 and BPHYT_RS10680 are separated by 103 nucleotidesBPHYT_RS10680 and BPHYT_RS10685 are separated by 21 nucleotides BPHYT_RS10665: BPHYT_RS10665 - muconolactone delta-isomerase, at 2,429,089 to 2,429,379 _RS10665 BPHYT_RS10670: BPHYT_RS10670 - catechol 1,2-dioxygenase, at 2,429,520 to 2,430,428 _RS10670 BPHYT_RS10675: BPHYT_RS10675 - muconate cycloisomerase, at 2,430,498 to 2,431,655 _RS10675 BPHYT_RS10680: BPHYT_RS10680 - LysR family transcriptional regulator, at 2,431,759 to 2,432,646 _RS10680 BPHYT_RS10685: BPHYT_RS10685 - LysR family transcriptional regulator, at 2,432,668 to 2,433,585 _RS10685
Group Condition BPHYT_RS10665 BPHYT_RS10670 BPHYT_RS10675 BPHYT_RS10680 BPHYT_RS10685
phage 36hr PHYTO2 MOI 1 N.D. +0.4 -1.8 -1.5 -0.9
nophagecontrol 36hr Phyto Lib Only N.D. +0.6 -4.3 +0.1 -0.2
phage 24hr PHYTO3 MOI 10 N.D. +0.0 -1.5 -2.4 +0.2
phage 36hr PHYTO10 MOI 1 N.D. +0.4 -1.0 -2.0 -0.6
phage P. phyto corn MOI 0.1 N.D. -0.0 -2.3 -0.5 -0.3
nophagecontrol 24hr Phyto Lib Only N.D. -0.1 -1.5 -0.6 -0.9
nitrogen source L-Lysine (N) N.D. -0.7 -2.0 -0.2 -0.2
carbon source Suberic (C) N.D. -1.1 -0.8 -0.9 -0.2
phage 24hr PHYTO11 MOI 0.1 N.D. -1.0 -0.8 -1.0 -0.0
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days N.D. -1.0 -1.0 -0.2 -0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -1.6 -0.6 -0.6 -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -2.2 -0.4 -0.2 +0.0
phage 36hr PHYTO11 MOI 1 N.D. -0.7 -1.5 -0.0 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. -1.2 +0.2 -0.7 -0.5
phage 36hr PHYTO2 MOI 1 N.D. +0.4 -0.9 -1.2 -0.5
carbon source adipate (C) N.D. -0.6 -0.9 -1.2 +0.5
phage 36hr PHYTO4 MOI 0.1 N.D. +0.7 +0.1 -2.4 -0.4
phage 24hr PHYTO2 MOI 1 N.D. +0.9 -0.2 -2.4 -0.3
vitamin No vitamins t1 N.D. -1.4 -0.9 +0.2 +0.5
phage 24hr PHYTO6 MOI 0.1 N.D. +0.8 -0.1 -1.7 -0.4
phage P. phyto PHYTO2 MOI 0.1 N.D. +0.8 -2.1 +0.3 -0.1
phage 24hr PHYTO1 MOI 1 N.D. +0.4 +0.8 -1.6 +0.1
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 N.D. -0.5 -0.9 +0.7 +0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. +0.5 -1.4 +1.0 +0.1
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.5 -0.8 +0.5 +1.3
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +1.6 -0.8 +0.3 +0.3
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days N.D. -0.8 +1.8 -0.5 +1.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. +0.4 +0.9 +1.0 -0.5
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days N.D. -0.6 +2.1 +0.1 +0.8
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days N.D. -0.1 +2.3 +0.5 +1.1
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