Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS10635 and BPHYT_RS10640 are separated by 123 nucleotidesBPHYT_RS10640 and BPHYT_RS10645 are separated by 39 nucleotidesBPHYT_RS10645 and BPHYT_RS10650 are separated by 197 nucleotidesBPHYT_RS10650 and BPHYT_RS10655 are separated by 169 nucleotides BPHYT_RS10635: BPHYT_RS10635 - transcriptional regulator, at 2,423,710 to 2,424,624 _RS10635 BPHYT_RS10640: BPHYT_RS10640 - carbonic anhydrase, at 2,424,748 to 2,425,407 _RS10640 BPHYT_RS10645: BPHYT_RS10645 - cyanate hydratase, at 2,425,447 to 2,425,917 _RS10645 BPHYT_RS10650: BPHYT_RS10650 - membrane protein, at 2,426,115 to 2,426,846 _RS10650 BPHYT_RS10655: BPHYT_RS10655 - LysR family transcriptional regulator, at 2,427,016 to 2,427,900 _RS10655
Group Condition BPHYT_RS10635 BPHYT_RS10640 BPHYT_RS10645 BPHYT_RS10650 BPHYT_RS10655
nophagecontrol 24hr Phyto Lib only +0.9 N.D. -3.4 -4.0 N.D.
phage P. phyto PHYTO2 MOI 1 -0.1 N.D. -3.5 -2.3 N.D.
phage 36hr PHYTO2 MOI 1 -0.1 N.D. -2.9 -1.0 N.D.
phage 36hr PHYTO9 MOI 0.1 +0.3 N.D. -0.8 -3.6 N.D.
phage 36hr PHYTO6 MOI 10 -0.4 N.D. -2.8 -0.6 N.D.
phage 24hr PHYTO3 MOI 0.1 -0.6 N.D. +0.2 -3.4 N.D.
phage PHYTO3 MOI 0.1 -0.2 N.D. -1.9 -1.5 N.D.
phage 36hr PHYTO6 MOI 1 +0.1 N.D. -1.6 -2.0 N.D.
phage 36hr PHYTO8 MOI 10 -1.4 N.D. -0.2 -1.8 N.D.
phage 24hr PHYTO6 MOI 0.1 -0.1 N.D. +0.3 -3.5 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 N.D. -0.4 -2.7 N.D.
phage 24hr PHYTO2 MOI 0.1 -0.2 N.D. -3.1 +0.1 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.0 N.D. -1.1 -2.1 N.D.
nophagecontrol 36hr Phyto Lib only -0.2 N.D. -3.0 +0.1 N.D.
nophagecontrol 24hr Phyto Lib Only -0.1 N.D. +0.7 -3.5 N.D.
phage 36hr PHYTO7 MOI 1 -0.2 N.D. +0.6 -3.3 N.D.
phage P. phyto PHYTO2 MOI 0.1 -0.1 N.D. +1.2 -3.9 N.D.
nitrogen source L-Phenylalanine (N) +0.2 N.D. -2.3 -0.7 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.3 N.D. -1.8 -1.0 N.D.
nophagecontrol 36hr Phyto Lib only +0.7 N.D. +0.2 -3.5 N.D.
phage 36hr PHYTO2 MOI 1 +0.9 N.D. -3.0 -0.4 N.D.
phage 24hr PHYTO3 MOI 10 +0.4 N.D. -3.3 +0.5 N.D.
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days +0.6 N.D. -0.6 -1.9 N.D.
phage 36hr PHYTO10 MOI 1 +0.7 N.D. -1.8 -0.7 N.D.
nitrogen source Ammonium chloride (N) +0.1 N.D. -2.5 +0.7 N.D.
motility outer cut, LB soft agar motility assay -0.8 N.D. +1.3 -1.4 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.7 N.D. -1.2 +1.1 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days -1.3 N.D. +1.6 -0.9 N.D.
phage 36hr PHYTO1 MOI 1 +0.1 N.D. +1.6 -1.4 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +2.1 N.D. +0.5 -0.5 N.D.
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