Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS10610 and BPHYT_RS10615 are separated by 36 nucleotidesBPHYT_RS10615 and BPHYT_RS10620 are separated by 56 nucleotidesBPHYT_RS10620 and BPHYT_RS10625 are separated by 119 nucleotidesBPHYT_RS10625 and BPHYT_RS10630 are separated by 108 nucleotides BPHYT_RS10610: BPHYT_RS10610 - LysR family transcriptional regulator, at 2,418,493 to 2,419,389 _RS10610 BPHYT_RS10615: BPHYT_RS10615 - phosphonoacetaldehyde hydrolase, at 2,419,426 to 2,420,229 _RS10615 BPHYT_RS10620: BPHYT_RS10620 - 2-aminoethylphosphonate:pyruvate aminotransferase, at 2,420,286 to 2,421,518 _RS10620 BPHYT_RS10625: BPHYT_RS10625 - LysR family transcriptional regulator, at 2,421,638 to 2,422,546 _RS10625 BPHYT_RS10630: BPHYT_RS10630 - oxidoreductase, at 2,422,655 to 2,423,401 _RS10630
Group Condition BPHYT_RS10610 BPHYT_RS10615 BPHYT_RS10620 BPHYT_RS10625 BPHYT_RS10630
phage 24hr PHYTO6 MOI 10 -1.0 -1.0 -0.2 N.D. -1.1
phage 36hr PHYTO2 MOI 1 +0.3 -0.3 -0.4 N.D. -1.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.2 -0.6 -0.4 N.D. -0.8
motility outer cut, LB soft agar motility assay +0.1 -0.9 -0.6 N.D. -0.5
phage 24hr PHYTO3 MOI 10 +0.1 -0.2 +0.3 N.D. -1.8
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days -0.3 -0.2 -0.5 N.D. -0.5
phage 24hr PHYTO10 MOI 1 +0.3 -0.2 -0.9 N.D. -0.7
phage 36hr PHYTO2 MOI 1 -0.3 -0.1 +0.3 N.D. -1.4
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.4 -0.1 -0.2 N.D. -1.1
phage 24hr PHYTO9 MOI 10 +0.3 -1.0 +0.2 N.D. -0.5
supernatant control Vogels_fungal_media; pH 5.5 -0.3 -0.6 -0.6 N.D. +0.5
phage 36hr PHYTO10 MOI 10 +0.2 -0.3 +0.4 N.D. -1.3
phage 36hr PHYTO8 MOI 10 -0.4 +0.5 -0.3 N.D. -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.5 -0.2 -0.9 N.D. -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.6 -0.5 -0.5 N.D. -0.3
nophagecontrol 24hr Phyto Lib Only -0.2 -0.3 +0.6 N.D. -0.6
phage 36hr PHYTO4 MOI 0.1 -0.0 -0.1 +0.6 N.D. -0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -1.1 +0.3 +0.5 N.D. +0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X +0.6 -0.5 -0.4 N.D. +0.1
carbon source Protocatechuic Acid (C) +0.4 +0.3 +0.1 N.D. -0.8
nophagecontrol 36hr Phyto Lib only +0.3 +0.0 +0.6 N.D. -0.8
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.8 +0.0 +0.2 N.D. -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.7 -0.1 -0.6 N.D. +0.6
phage 24hr PHYTO3 MOI 10 +0.4 +0.4 +0.5 N.D. -0.5
nitrogen source NAG (N) -0.0 -0.1 +0.6 N.D. +0.8
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.0 +1.3 +0.1 N.D. -0.9
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.1 +0.6 +0.4 N.D. +0.7
nitrogen source Ammonium chloride (N) +0.4 +0.3 +0.7 N.D. +0.3
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.7 +0.4 +0.2 N.D. +0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.7 +0.0 +0.2 N.D. +0.9
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