Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS10460 and BPHYT_RS10465 are separated by 482 nucleotidesBPHYT_RS10465 and BPHYT_RS10470 are separated by 22 nucleotidesBPHYT_RS10470 and BPHYT_RS10475 are separated by 163 nucleotidesBPHYT_RS10475 and BPHYT_RS10480 are separated by 6 nucleotides BPHYT_RS10460: BPHYT_RS10460 - endoribonuclease, at 2,377,721 to 2,378,164 _RS10460 BPHYT_RS10465: BPHYT_RS10465 - hypothetical protein, at 2,378,647 to 2,380,101 _RS10465 BPHYT_RS10470: BPHYT_RS10470 - hypothetical protein, at 2,380,124 to 2,381,086 _RS10470 BPHYT_RS10475: BPHYT_RS10475 - mammalian cell entry protein, at 2,381,250 to 2,382,869 _RS10475 BPHYT_RS10480: BPHYT_RS10480 - LPS biosynthesis protein, at 2,382,876 to 2,385,161 _RS10480
Group Condition BPHYT_RS10460 BPHYT_RS10465 BPHYT_RS10470 BPHYT_RS10475 BPHYT_RS10480
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X N.D. -0.1 -2.1 -1.6 -0.7
supernatant control Vogels_fungal_media 0.1X N.D. -1.0 -1.7 -1.0 -0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. -1.1 -1.9 -1.0 +0.1
phage 36hr PHYTO3 MOI 1 N.D. +0.2 +1.1 -4.9 +0.1
carbon source pyruvate (C) N.D. -1.3 -1.0 -0.8 -0.3
phage 24hr PHYTO2 MOI 0.1 N.D. -0.3 -1.7 -0.9 -0.2
carbon source D-Lactate (C) N.D. -1.0 -1.1 -0.6 -0.2
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days N.D. -0.5 -1.1 -0.8 -0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. -0.5 -1.2 -0.6 -0.4
supernatant control Vogels_fungal_media; pH 5.5 N.D. -0.7 -1.5 -0.5 +0.3
phage 24hr PHYTO4 MOI 10 N.D. +0.6 -1.2 -1.0 -0.3
phage 24hr PHYTO10 MOI 1 N.D. +0.2 +0.3 -2.2 +0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. +0.4 +0.5 -2.8 +0.3
phage 36hr PHYTO9 MOI 1 N.D. +0.1 +1.0 -1.5 -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.2 -1.7 +0.3 +0.6
vitamin With vitamins t2 N.D. +0.4 -2.1 +1.0 +0.5
vitamin No vitamins t1 N.D. +0.5 -1.3 +0.5 +0.5
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days N.D. -0.1 +0.7 +1.5 +0.5
stress Cephalothin 2 mg/ml N.D. +1.1 +0.7 +0.7 +0.4
motility outer cut, LB soft agar motility assay N.D. +0.6 +0.5 +1.7 +0.2
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.5 +1.3 +1.4 -0.1
carbon source Tween 20 (C) N.D. +0.6 +1.0 +1.2 +0.4
motility inner cut, LB soft agar motility assay N.D. +1.0 +1.3 +0.2 +0.7
temperature LB, 37C N.D. +0.7 +1.2 +0.7 +0.6
motility outer cut, LB soft agar motility assay N.D. +0.7 +0.9 +1.1 +0.5
temperature LB, 23C N.D. +1.2 +1.0 +0.5 +0.6
temperature LB, 23C N.D. +1.1 +0.8 +0.8 +0.6
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +1.0 +0.7 +0.7 +1.0
stress Bacitracin 0.25 mg/ml N.D. +0.6 +1.3 +1.3 +0.2
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 N.D. +1.4 +1.0 +1.4 +0.5
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