Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS10430 and BPHYT_RS10435 are separated by 558 nucleotidesBPHYT_RS10435 and BPHYT_RS10440 are separated by 163 nucleotidesBPHYT_RS10440 and BPHYT_RS10445 are separated by 270 nucleotidesBPHYT_RS10445 and BPHYT_RS10450 are separated by 388 nucleotides BPHYT_RS10430: BPHYT_RS10430 - glycosyl transferase family 1, at 2,370,630 to 2,371,745 _RS10430 BPHYT_RS10435: BPHYT_RS10435 - ThiJ/PfpI domain-containing protein, at 2,372,304 to 2,372,921 _RS10435 BPHYT_RS10440: BPHYT_RS10440 - GntR family transcriptional regulator, at 2,373,085 to 2,374,419 _RS10440 BPHYT_RS10445: BPHYT_RS10445 - hypothetical protein, at 2,374,690 to 2,375,439 _RS10445 BPHYT_RS10450: BPHYT_RS10450 - prolyl-tRNA synthetase, at 2,375,828 to 2,376,970 _RS10450
Group Condition BPHYT_RS10430 BPHYT_RS10435 BPHYT_RS10440 BPHYT_RS10445 BPHYT_RS10450
carbon source 2-Deoxy-D-ribonic lithium salt (C) -1.7 -1.1 +0.5 N.D. N.D.
supernatant control Vogels_fungal_media 0.1X -0.3 -1.7 -0.3 N.D. N.D.
no phage control No phage control +0.2 -1.0 -1.1 N.D. N.D.
carbon source Malonic (C) +0.2 -1.1 -0.5 N.D. N.D.
supernatant control Vogels_fungal_media; pH 5.5 +0.5 -0.6 -0.9 N.D. N.D.
phage 36hr PHYTO2 MOI 1 -0.2 +0.5 -1.0 N.D. N.D.
nitrogen source L-Glutamic (N) +0.5 -1.5 +0.3 N.D. N.D.
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -0.2 +0.8 -1.2 N.D. N.D.
phage 36hr PHYTO7 MOI 1 +0.2 +0.4 -1.1 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.8 -0.2 -0.8 N.D. N.D.
phage P. phyto PHYTO2 MOI 1 +1.2 -0.1 -1.2 N.D. N.D.
phage 24hr PHYTO2 MOI 1 -0.2 +0.8 -0.7 N.D. N.D.
phage 24hr PHYTO11 MOI 1 +1.0 -0.4 -0.6 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.7 +0.4 +0.6 N.D. N.D.
phage 36hr PHYTO1 MOI 10 +0.7 +0.6 -0.5 N.D. N.D.
carbon source Suberic (C) +1.0 +0.5 -0.5 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.9 -0.4 +0.6 N.D. N.D.
phage 24hr PHYTO8 MOI 1 +0.7 +1.0 -0.4 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +2.0 +0.1 -0.2 N.D. N.D.
motility inner cut, LB soft agar motility assay +0.6 +0.6 +0.6 N.D. N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +1.2 +0.1 +0.5 N.D. N.D.
temperature LB, 23C +0.4 +0.5 +0.9 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.9 +0.8 +0.2 N.D. N.D.
motility inner cut, LB soft agar motility assay +0.8 +0.6 +0.6 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.7 +1.0 +0.3 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.5 +1.0 +0.5 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +1.4 +0.2 +0.5 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +1.2 -0.2 +1.1 N.D. N.D.
lb LB +0.4 +0.8 +1.0 N.D. N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.8 +0.9 +0.6 N.D. N.D.
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