Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS10415 and BPHYT_RS10420 are separated by 432 nucleotidesBPHYT_RS10420 and BPHYT_RS10425 are separated by 60 nucleotidesBPHYT_RS10425 and BPHYT_RS10430 overlap by 4 nucleotidesBPHYT_RS10430 and BPHYT_RS10435 are separated by 558 nucleotides BPHYT_RS10415: BPHYT_RS10415 - taurine dioxygenase, at 2,366,809 to 2,367,756 _RS10415 BPHYT_RS10420: BPHYT_RS10420 - phospholipase, at 2,368,189 to 2,369,742 _RS10420 BPHYT_RS10425: BPHYT_RS10425 - polysaccharide deacetylase, at 2,369,803 to 2,370,633 _RS10425 BPHYT_RS10430: BPHYT_RS10430 - glycosyl transferase family 1, at 2,370,630 to 2,371,745 _RS10430 BPHYT_RS10435: BPHYT_RS10435 - ThiJ/PfpI domain-containing protein, at 2,372,304 to 2,372,921 _RS10435
Group Condition BPHYT_RS10415 BPHYT_RS10420 BPHYT_RS10425 BPHYT_RS10430 BPHYT_RS10435
carbon source 2-Deoxy-D-ribonic lithium salt (C) -0.5 +0.0 -0.0 -1.7 -1.1
phage 36hr PHYTO2 MOI 1 -0.5 -0.8 -0.9 -0.8 +0.2
supernatant control Vogels_fungal_media 0.1X -0.3 -0.3 -0.1 -0.3 -1.7
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.2 -0.3 -0.7 -0.6 -0.4
carbon source Phenylacetic 5 mM (C) -0.6 -0.6 -0.9 -0.3 +0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days +0.4 -0.2 -0.2 -0.8 -0.6
phage P. phyto PHYTO2 MOI 0.1 +0.1 -0.2 -1.4 +0.7 -0.6
nitrogen source L-Glutamic (N) -0.1 +0.5 -0.4 +0.5 -1.5
carbon source Malonic (C) +0.4 -0.4 +0.2 +0.2 -1.1
nitrogen source L-Isoleucine (N) +0.1 -0.3 -0.7 +0.9 -0.4
phage 24hr PHYTO3 MOI 10 -0.3 -0.7 +0.5 -0.4 +0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.2 +0.4 -0.7 +0.4 +0.8
carbon source Suberic (C) -0.3 +0.6 -0.1 +0.9 -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 +0.6 -0.2 +0.9 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.4 +0.1 -0.1 +0.7 +1.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.3 +0.5 +0.6 +0.7 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.0 -0.7 +0.5 +1.4 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.1 -0.3 +0.7 +0.4 +1.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.4 +0.2 -0.2 +1.0 +0.4
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.3 -0.5 +0.9 +1.2 +0.1
temperature LB, 23C +0.2 +0.3 +0.8 +0.4 +0.5
carbon source Suberic (C) +0.4 +0.7 -0.3 +1.0 +0.5
phage 24hr PHYTO8 MOI 1 +0.1 +0.4 +0.1 +0.7 +1.0
motility outer cut, LB soft agar motility assay +0.3 +0.2 +0.7 +0.6 +0.5
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.0 -0.1 +0.8 +0.8 +0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.4 +0.1 +0.4 +0.5 +1.0
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 -0.0 +0.9 +0.9 +0.8
motility inner cut, LB soft agar motility assay +0.1 +0.5 +0.7 +0.8 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.8 +0.7 +0.1 +1.2 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.4 -0.2 +0.8 +2.0 +0.1
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