Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS10195 and BPHYT_RS10200 are separated by 490 nucleotidesBPHYT_RS10200 and BPHYT_RS10205 are separated by 43 nucleotidesBPHYT_RS10205 and BPHYT_RS10210 are separated by 151 nucleotidesBPHYT_RS10210 and BPHYT_RS10215 are separated by 88 nucleotides BPHYT_RS10195: BPHYT_RS10195 - major facilitator transporter, at 2,321,353 to 2,322,690 _RS10195 BPHYT_RS10200: BPHYT_RS10200 - short-chain dehydrogenase, at 2,323,181 to 2,323,561 _RS10200 BPHYT_RS10205: BPHYT_RS10205 - D-lactate dehydrogenase, at 2,323,605 to 2,325,344 _RS10205 BPHYT_RS10210: BPHYT_RS10210 - LysR family transcriptional regulator, at 2,325,496 to 2,326,395 _RS10210 BPHYT_RS10215: BPHYT_RS10215 - sulfate transporter, at 2,326,484 to 2,326,954 _RS10215
Group Condition BPHYT_RS10195 BPHYT_RS10200 BPHYT_RS10205 BPHYT_RS10210 BPHYT_RS10215
phage 24hr PHYTO4 MOI 0.1 -0.2 N.D. -0.4 N.D. -3.0
phage P. phyto corn MOI 0.1 -0.2 N.D. -0.4 N.D. -2.7
phage 24hr PHYTO9 MOI 0.1 -0.8 N.D. -0.6 N.D. -1.7
phage 36hr PHYTO11 MOI 0.1 -0.2 N.D. +0.2 N.D. -3.2
phage 24hr PHYTO10 MOI 10 +0.5 N.D. -0.3 N.D. -3.1
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -0.5 N.D. -1.0 N.D. -1.3
phage 24hr PHYTO11 MOI 1 -0.2 N.D. -1.4 N.D. -1.0
nophagecontrol 24hr Phyto Lib Only -0.1 N.D. -0.7 N.D. -1.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.4 N.D. -0.2 N.D. -2.5
phage 24hr PHYTO3 MOI 10 -0.3 N.D. -0.1 N.D. -1.7
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -0.1 N.D. +0.2 N.D. -2.2
phage P. phyto PHYTO2 MOI 0.1 +0.2 N.D. -0.2 N.D. -2.0
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.4 N.D. +0.5 N.D. -2.1
phage 36hr PHYTO4 MOI 0.1 -0.6 N.D. +0.4 N.D. -1.5
phage 24hr PHYTO3 MOI 10 +0.2 N.D. -0.8 N.D. -1.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.5 N.D. -0.6 N.D. -1.6
nophagecontrol 24hr Phyto Lib Only -0.1 N.D. +0.3 N.D. -1.7
supernatant control Vogels_fungal_media 0.1X +0.4 N.D. +0.0 N.D. -1.8
in planta Plant=Brachypodium_distachyon ; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.6 N.D. +0.4 N.D. -1.1
phage 24hr PHYTO MOI 0.1 +0.3 N.D. +0.4 N.D. -1.5
carbon source 2-Deoxy-D-ribonic lithium salt (C) +0.8 N.D. +0.1 N.D. -1.5
nitrogen source L-Glutamic (N) -1.2 N.D. -0.1 N.D. +1.0
phage 24hr PHYTO6 MOI 10 -0.8 N.D. +1.6 N.D. -0.4
phage 36hr PHYTO8 MOI 10 -0.8 N.D. +0.4 N.D. +1.0
phage 36hr PHYTO3 MOI 1 -0.5 N.D. -0.2 N.D. +1.4
phage 24hr PHYTO3 MOI 0.1 -0.6 N.D. -0.1 N.D. +1.4
nitrogen source L-Proline (N) -0.7 N.D. +0.2 N.D. +1.2
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -0.1 N.D. +1.9 N.D. -0.9
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.2 N.D. +2.0 N.D. -1.2
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -0.1 N.D. +2.3 N.D. -0.1
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