Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS10185 and BPHYT_RS10190 are separated by 176 nucleotidesBPHYT_RS10190 and BPHYT_RS10195 are separated by 76 nucleotidesBPHYT_RS10195 and BPHYT_RS10200 are separated by 490 nucleotidesBPHYT_RS10200 and BPHYT_RS10205 are separated by 43 nucleotides BPHYT_RS10185: BPHYT_RS10185 - MarR family transcriptional regulator, at 2,319,031 to 2,319,528 _RS10185 BPHYT_RS10190: BPHYT_RS10190 - phosphonate monoester hydrolase, at 2,319,705 to 2,321,276 _RS10190 BPHYT_RS10195: BPHYT_RS10195 - major facilitator transporter, at 2,321,353 to 2,322,690 _RS10195 BPHYT_RS10200: BPHYT_RS10200 - short-chain dehydrogenase, at 2,323,181 to 2,323,561 _RS10200 BPHYT_RS10205: BPHYT_RS10205 - D-lactate dehydrogenase, at 2,323,605 to 2,325,344 _RS10205
Group Condition BPHYT_RS10185 BPHYT_RS10190 BPHYT_RS10195 BPHYT_RS10200 BPHYT_RS10205
phage 24hr PHYTO6 MOI 1 -2.2 -0.6 -0.7 N.D. +0.3
phage 36hr PHYTO1 MOI 1 -1.5 -0.7 -0.3 N.D. -0.6
phage 36hr PHYTO4 MOI 1 -1.7 -0.2 -0.5 N.D. -0.4
phage 36hr PHYTO1 MOI 10 -0.9 -1.1 -0.5 N.D. -0.0
phage 24hr PHYTO MOI 0.1 -2.9 -0.1 -0.2 N.D. +0.8
nophagecontrol 36hr Phyto Lib only -0.7 -0.1 -0.7 N.D. -0.9
phage 24hr PHYTO11 MOI 1 -0.9 +0.2 -0.2 N.D. -1.4
phage 24hr PHYTO2 MOI 1 -1.9 -0.3 +0.0 N.D. +0.0
phage P. phyto corn MOI 1 -1.7 -0.2 -0.2 N.D. +0.0
nophagecontrol 24hr Phyto Lib Only -1.0 -0.2 -0.1 N.D. -0.7
phage 36hr PHYTO10 MOI 10 -0.3 -0.2 -0.2 N.D. -1.3
phage 24hr PHYTO8 MOI 1 -1.2 -0.1 -0.4 N.D. -0.3
carbon source Suberic (C) -0.5 -0.0 -0.2 N.D. -1.2
phage 36hr PHYTO11 MOI 1 +0.2 -0.5 -0.4 N.D. -0.9
phage 36hr PHYTO9 MOI 1 -1.0 -0.8 -0.4 N.D. +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -1.3 +0.2 +0.3 N.D. -0.5
phage 36hr PHYTO2 MOI 1 -1.4 -0.3 +0.1 N.D. +0.4
phage 24hr PHYTO6 MOI 0.1 -1.0 -0.1 -0.7 N.D. +0.4
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days +0.5 -0.0 -0.5 N.D. -1.0
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.8 -0.4 -0.4 N.D. +0.5
carbon source D-Lactate (C) +0.6 -0.0 +0.0 N.D. -1.4
phage 24hr PHYTO9 MOI 0.1 +0.7 +0.0 -0.8 N.D. -0.6
nophagecontrol 24hr Phyto Lib only +0.7 +0.2 -0.2 N.D. -1.4
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 +0.7 -0.4 -0.4 N.D. +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.7 -0.1 -0.4 N.D. +0.8
phage 24hr PHYTO6 MOI 10 +0.9 +0.1 -0.8 N.D. +1.6
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -0.2 +0.3 -0.1 N.D. +1.9
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -0.3 +0.2 -0.1 N.D. +2.3
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -0.4 +0.4 +0.2 N.D. +2.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.9 +0.0 +0.2 N.D. +1.3
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