Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS10055 and BPHYT_RS10060 are separated by 83 nucleotidesBPHYT_RS10060 and BPHYT_RS10065 are separated by 1 nucleotidesBPHYT_RS10065 and BPHYT_RS10070 are separated by 44 nucleotidesBPHYT_RS10070 and BPHYT_RS10075 are separated by 98 nucleotides BPHYT_RS10055: BPHYT_RS10055 - cytochrome C, at 2,289,551 to 2,290,591 _RS10055 BPHYT_RS10060: BPHYT_RS10060 - cytochrome C oxidase, at 2,290,675 to 2,292,660 _RS10060 BPHYT_RS10065: BPHYT_RS10065 - cytochrome C oxidase subunit I, at 2,292,662 to 2,293,306 _RS10065 BPHYT_RS10070: BPHYT_RS10070 - hypothetical protein, at 2,293,351 to 2,293,734 _RS10070 BPHYT_RS10075: BPHYT_RS10075 - alcohol dehydrogenase, at 2,293,833 to 2,295,134 _RS10075
Group Condition BPHYT_RS10055 BPHYT_RS10060 BPHYT_RS10065 BPHYT_RS10070 BPHYT_RS10075
carbon source L-Fucose (C) N.D. +0.1 -4.9 -0.2 -0.3
carbon source Phenylacetic 5 mM (C) N.D. -0.1 -4.1 -0.5 +0.9
phage P. phyto PHYTO2 MOI 1 N.D. -0.1 -2.6 -0.0 -0.8
stress Paraquat dichloride 0.064 mg/ml N.D. -0.0 -2.8 -0.4 +0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X N.D. +0.0 -1.2 -1.4 -0.6
phage 36hr PHYTO7 MOI 0.1 N.D. -0.3 -1.9 +0.1 -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. +0.1 -2.5 -0.4 +0.1
stress Bacitracin 0.25 mg/ml N.D. +0.1 -2.8 -0.3 +0.1
carbon source D-Ribose (C) N.D. +0.0 -1.9 -0.6 -0.2
phage P. phyto PHYTO2 MOI 1 N.D. -0.8 -2.2 +0.7 -0.2
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. -0.3 -2.6 -0.0 +0.4
phage 36hr PHYTO1 MOI 10 N.D. -0.4 -1.7 +0.2 -0.6
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. -0.2 -2.3 -0.1 +0.6
nophagecontrol 36hr Phyto Lib Only N.D. -0.1 -1.9 +0.6 -0.5
phage 24hr PHYTO10 MOI 10 N.D. -0.6 -1.5 +0.4 -0.3
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days N.D. +0.4 -1.9 -0.2 -0.3
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days N.D. +0.2 -1.5 +0.4 +0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -0.1 -2.0 +1.2 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. -0.2 +1.4 -0.6 -0.8
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -0.0 -1.3 +1.5 +0.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -0.2 -0.8 +1.3 +0.6
phage 36hr PHYTO10 MOI 10 N.D. -0.8 +2.1 +0.4 -0.7
phage 36hr PHYTO2 MOI 1 N.D. -0.2 +1.7 +0.6 -1.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -0.3 -1.5 +1.7 +1.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -0.1 -1.6 +2.5 +0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -0.1 -0.7 +1.7 +0.4
phage 36hr PHYTO11 MOI 1 N.D. -0.5 +1.7 +0.4 -0.3
phage 36hr PHYTO3 MOI 0.1 N.D. -0.5 +2.2 +0.2 -0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -0.3 +0.4 +1.9 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. +0.9 +0.8 -0.8 +2.0
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