Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS09545 and BPHYT_RS09550 are separated by 130 nucleotidesBPHYT_RS09550 and BPHYT_RS09555 are separated by 193 nucleotidesBPHYT_RS09555 and BPHYT_RS09560 are separated by 160 nucleotidesBPHYT_RS09560 and BPHYT_RS09565 are separated by 88 nucleotides BPHYT_RS09545: BPHYT_RS09545 - peptide methionine sulfoxide reductase, at 2,164,886 to 2,165,308 _RS09545 BPHYT_RS09550: BPHYT_RS09550 - hypothetical protein, at 2,165,439 to 2,166,995 _RS09550 BPHYT_RS09555: BPHYT_RS09555 - long-chain fatty acid--CoA ligase, at 2,167,189 to 2,168,874 _RS09555 BPHYT_RS09560: BPHYT_RS09560 - 3-hydroxyacyl-CoA dehydrogenase, at 2,169,035 to 2,171,119 _RS09560 BPHYT_RS09565: BPHYT_RS09565 - acetyl-CoA acetyltransferase, at 2,171,208 to 2,172,386 _RS09565
Group Condition BPHYT_RS09545 BPHYT_RS09550 BPHYT_RS09555 BPHYT_RS09560 BPHYT_RS09565
carbon source Sebacic (C) -0.5 +0.2 -0.7 -1.9 -3.5
carbon source Azelaic (C) +0.0 +0.3 -0.0 -2.8 -3.7
carbon source Dodecandioic (C) -0.5 +0.1 -0.4 -2.4 -3.0
nophagecontrol 36hr Phyto Lib only -3.6 +0.2 +0.0 -0.7 -1.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -2.1 -0.4 -0.2 -0.3 -1.7
phage P. phyto PHYTO2 MOI 1 -2.0 -0.2 -0.1 +0.7 -2.7
phage 24hr PHYTO2 MOI 1 -3.8 -0.2 -0.4 +0.2 +0.7
phage 36hr PHYTO8 MOI 10 -3.5 -0.1 -0.1 -0.1 +0.2
phage 24hr PHYTO6 MOI 10 +0.6 -0.2 -0.2 -0.4 -3.1
nitrogen source L-Isoleucine (N) -2.6 +0.1 -0.1 +0.2 -0.5
carbon source Tween 20 (C) -2.3 +0.2 +0.4 -0.3 -1.0
phage 24hr PHYTO11 MOI 0.1 -0.6 +0.2 +0.1 -0.4 -1.9
phage 36hr PHYTO4 MOI 1 -1.6 +0.0 +0.1 -0.5 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.0 -0.4 +0.2 -0.4 -2.0
nophagecontrol 36hr Phyto Lib only -1.7 -0.3 -0.4 -0.8 +0.6
phage P. phyto PHYTO2 MOI 1 +0.9 +0.0 -0.3 -0.0 -3.0
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 -2.2 +0.1 +0.3 -0.9 +0.5
carbon source Sebacic (C) +0.2 +0.7 -0.4 -1.9 -0.5
carbon source Suberic (C) -1.2 +0.5 -1.4 -0.4 +0.6
phage 24hr PHYTO3 MOI 10 +1.0 +0.3 +0.2 +0.1 -3.5
supernatant control Vogels_fungal_media 0.1X -1.9 +0.0 -0.1 +0.0 +0.7
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.8 +0.1 -0.2 -0.8 +1.0
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -1.3 -0.1 -0.2 +0.1 +1.1
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days -1.3 +0.1 -0.1 -0.1 +1.3
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) +1.1 -0.5 +0.2 -1.4 +0.7
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB -0.2 -0.3 -0.4 -0.3 +1.7
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +2.5 +0.1 +0.2 -0.4 -0.4
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +2.0 -0.2 +0.2 -0.2 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.6 -0.1 +0.3 -0.6 +2.2
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent +1.0 -0.1 +0.2 +0.6 +0.8
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