Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS09525 and BPHYT_RS09530 are separated by 75 nucleotidesBPHYT_RS09530 and BPHYT_RS09535 are separated by 11 nucleotidesBPHYT_RS09535 and BPHYT_RS09540 are separated by 1 nucleotidesBPHYT_RS09540 and BPHYT_RS09545 are separated by 163 nucleotides BPHYT_RS09525: BPHYT_RS09525 - toxin HipA, at 2,161,711 to 2,163,003 _RS09525 BPHYT_RS09530: BPHYT_RS09530 - peptidyl-prolyl cis-trans isomerase, at 2,163,079 to 2,163,858 _RS09530 BPHYT_RS09535: BPHYT_RS09535 - BolA family transcriptional regulator, at 2,163,870 to 2,164,190 _RS09535 BPHYT_RS09540: BPHYT_RS09540 - intracellular septation protein A, at 2,164,192 to 2,164,722 _RS09540 BPHYT_RS09545: BPHYT_RS09545 - peptide methionine sulfoxide reductase, at 2,164,886 to 2,165,308 _RS09545
Group Condition BPHYT_RS09525 BPHYT_RS09530 BPHYT_RS09535 BPHYT_RS09540 BPHYT_RS09545
phage 24hr PHYTO2 MOI 1 -0.4 N.D. -0.4 N.D. -3.8
nophagecontrol 36hr Phyto Lib only +0.2 N.D. -0.4 N.D. -3.6
phage 36hr PHYTO8 MOI 10 +0.4 N.D. -0.5 N.D. -3.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.6 N.D. -0.3 N.D. -2.1
nitrogen source L-Isoleucine (N) -0.3 N.D. -0.1 N.D. -2.6
phage P. phyto corn MOI 0.1 -1.0 N.D. -0.7 N.D. -1.2
phage P. phyto corn MOI 1 -0.9 N.D. -0.3 N.D. -1.4
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -0.8 N.D. -0.3 N.D. -1.3
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.9 N.D. -0.6 N.D. -0.8
supernatant control Vogels_fungal_media 0.1X -0.4 N.D. +0.1 N.D. -1.9
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) -0.8 N.D. -0.5 N.D. -0.8
carbon source Tween 20 (C) -0.3 N.D. +0.5 N.D. -2.3
phage P. phyto PHYTO2 MOI 1 +0.4 N.D. -0.5 N.D. -2.0
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 +0.5 N.D. -0.4 N.D. -2.2
phage 24hr PHYTO6 MOI 1 -2.4 N.D. +0.4 N.D. +0.3
nophagecontrol 36hr Phyto Lib only -0.3 N.D. +0.5 N.D. -1.7
phage 36hr PHYTO7 MOI 0.1 -1.6 N.D. +0.5 N.D. -0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 N.D. +0.8 N.D. -1.8
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB -0.8 N.D. +0.6 N.D. -0.9
phage 36hr PHYTO4 MOI 1 +0.2 N.D. +0.6 N.D. -1.6
nophagecontrol 36hr Phyto Lib Only +0.9 N.D. +0.0 N.D. -1.6
phage P. phyto PHYTO2 MOI 1 -1.2 N.D. -0.3 N.D. +0.9
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) -0.7 N.D. -0.7 N.D. +1.1
nitrogen source L-Lysine (N) +1.0 N.D. -0.7 N.D. -0.6
phage 36hr PHYTO MOI 0.1 +0.4 N.D. +0.6 N.D. -1.2
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 +1.7 N.D. -0.4 N.D. +0.0
carbon source D-Glucose 20 mM (C) -0.4 N.D. +0.4 N.D. +1.4
phage 24hr PHYTO3 MOI 10 +0.9 N.D. -0.5 N.D. +1.0
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.1 N.D. +0.4 N.D. +2.0
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.3 N.D. +0.4 N.D. +2.5
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