Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS09285 and BPHYT_RS09290 are separated by 154 nucleotidesBPHYT_RS09290 and BPHYT_RS09295 are separated by 121 nucleotidesBPHYT_RS09295 and BPHYT_RS09300 are separated by 46 nucleotidesBPHYT_RS09300 and BPHYT_RS09305 are separated by 87 nucleotides BPHYT_RS09285: BPHYT_RS09285 - acetylornithine deacetylase, at 2,103,355 to 2,104,569 _RS09285 BPHYT_RS09290: BPHYT_RS09290 - helicase, at 2,104,724 to 2,108,206 _RS09290 BPHYT_RS09295: BPHYT_RS09295 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 2,108,328 to 2,109,044 _RS09295 BPHYT_RS09300: BPHYT_RS09300 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 2,109,091 to 2,109,570 _RS09300 BPHYT_RS09305: BPHYT_RS09305 - alkyl hydroperoxide reductase, at 2,109,658 to 2,110,206 _RS09305
Group Condition BPHYT_RS09285 BPHYT_RS09290 BPHYT_RS09295 BPHYT_RS09300 BPHYT_RS09305
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.4 -0.7 N.D. N.D. -1.0
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.6 -0.6 N.D. N.D. -0.6
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.7 -0.5 N.D. N.D. -0.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.4 -0.6 N.D. N.D. -0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.8 -0.5 N.D. N.D. -0.4
nitrogen source L-Proline (N) -0.5 -0.6 N.D. N.D. -0.5
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -0.2 -0.6 N.D. N.D. -0.6
motility outer cut, LB soft agar motility assay +0.1 -1.0 N.D. N.D. -0.3
carbon source Phenylacetic 5 mM (C) +0.2 -0.5 N.D. N.D. -0.9
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) +0.2 -0.5 N.D. N.D. -0.8
phage 24hr PHYTO9 MOI 10 -1.1 -0.1 N.D. N.D. +0.2
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days -0.3 -0.9 N.D. N.D. +0.3
carbon source succinate (C) +0.3 -0.6 N.D. N.D. -0.5
motility inner cut, LB soft agar motility assay +0.3 -0.8 N.D. N.D. -0.3
motility outer cut, LB soft agar motility assay +0.2 -1.1 N.D. N.D. +0.3
phage 36hr PHYTO6 MOI 0.1 +0.5 -0.1 N.D. N.D. -1.0
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days -0.5 -0.6 N.D. N.D. +0.5
phage 36hr PHYTO3 MOI 1 -0.2 +0.4 N.D. N.D. -0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.5 -0.9 N.D. N.D. -0.1
phage 24hr PHYTO11 MOI 0.1 +0.5 -0.2 N.D. N.D. -0.8
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.7 -0.9 N.D. N.D. -0.2
motility outer cut, LB soft agar motility assay +0.5 -0.9 N.D. N.D. +0.0
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.4 +0.6 N.D. N.D. -0.4
phage 24hr PHYTO2 MOI 0.1 +0.9 -0.2 N.D. N.D. -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 -0.5 N.D. N.D. +0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +1.3 -0.5 N.D. N.D. -0.2
phage 24hr PHYTO3 MOI 10 +0.5 +0.8 N.D. N.D. -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.9 -0.2 N.D. N.D. +0.4
phage 36hr PHYTO8 MOI 10 +0.5 +0.9 N.D. N.D. -0.2
phage 24hr PHYTO7 MOI 10 +0.6 +0.4 N.D. N.D. +0.4
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