Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS09280 and BPHYT_RS09285 are separated by 54 nucleotidesBPHYT_RS09285 and BPHYT_RS09290 are separated by 154 nucleotidesBPHYT_RS09290 and BPHYT_RS09295 are separated by 121 nucleotidesBPHYT_RS09295 and BPHYT_RS09300 are separated by 46 nucleotides BPHYT_RS09280: BPHYT_RS09280 - pyridoxal-5'-phosphate-dependent protein subunit beta, at 2,102,266 to 2,103,300 _RS09280 BPHYT_RS09285: BPHYT_RS09285 - acetylornithine deacetylase, at 2,103,355 to 2,104,569 _RS09285 BPHYT_RS09290: BPHYT_RS09290 - helicase, at 2,104,724 to 2,108,206 _RS09290 BPHYT_RS09295: BPHYT_RS09295 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 2,108,328 to 2,109,044 _RS09295 BPHYT_RS09300: BPHYT_RS09300 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 2,109,091 to 2,109,570 _RS09300
Group Condition BPHYT_RS09280 BPHYT_RS09285 BPHYT_RS09290 BPHYT_RS09295 BPHYT_RS09300
phage 24hr PHYTO8 MOI 1 -3.4 +0.0 +0.0 N.D. N.D.
phage 24hr PHYTO9 MOI 10 -1.9 -1.1 -0.1 N.D. N.D.
phage 24hr PHYTO11 MOI 1 -3.5 +0.3 +0.0 N.D. N.D.
phage 24hr PHYTO3 MOI 10 -2.9 +0.2 -0.0 N.D. N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -1.5 -0.7 -0.5 N.D. N.D.
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent -2.4 -0.1 -0.0 N.D. N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -1.5 -0.8 -0.2 N.D. N.D.
phage 36hr PHYTO2 MOI 0.1 -2.7 +0.2 -0.0 N.D. N.D.
vitamin With vitamins t1 -2.0 -0.3 -0.1 N.D. N.D.
phage 24hr PHYTO4 MOI 0.1 -2.6 -0.2 +0.3 N.D. N.D.
phage 36hr PHYTO10 MOI 1 -2.8 +0.1 +0.2 N.D. N.D.
phage 36hr PHYTO6 MOI 0.1 -2.6 +0.5 -0.3 N.D. N.D.
phage 24hr PHYTO6 MOI 1 -2.6 +0.4 -0.0 N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB -2.1 +0.0 -0.2 N.D. N.D.
phage 36hr PHYTO9 MOI 1 -2.8 +0.5 +0.1 N.D. N.D.
carbon source Sebacic (C) -2.0 +0.2 -0.2 N.D. N.D.
carbon source Suberic (C) -2.3 +0.3 +0.1 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -2.1 -0.4 +0.6 N.D. N.D.
phage 36hr PHYTO7 MOI 0.1 -2.8 +0.7 +0.3 N.D. N.D.
phage 36hr PHYTO4 MOI 0.1 -2.6 +0.6 +0.1 N.D. N.D.
no phage control No phage control -1.8 -0.1 +0.4 N.D. N.D.
carbon source adipate (C) -1.1 +0.7 -0.6 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.7 +0.7 -0.9 N.D. N.D.
phage P. phyto corn MOI 1 -1.8 +0.3 +0.7 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.5 -0.1 -0.8 N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +1.7 +0.3 -0.6 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +2.7 +0.1 -0.5 N.D. N.D.
phage 36hr PHYTO11 MOI 0.1 +1.5 +0.5 +0.3 N.D. N.D.
phage 36hr PHYTO2 MOI 1 +2.1 +0.4 -0.1 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +2.0 +1.3 -0.5 N.D. N.D.
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