Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS09225 and BPHYT_RS09230 are separated by 177 nucleotidesBPHYT_RS09230 and BPHYT_RS09235 are separated by 353 nucleotidesBPHYT_RS09235 and BPHYT_RS09240 are separated by 38 nucleotidesBPHYT_RS09240 and BPHYT_RS09245 overlap by 4 nucleotides BPHYT_RS09225: BPHYT_RS09225 - LysR family transcriptional regulator, at 2,088,891 to 2,089,832 _RS09225 BPHYT_RS09230: BPHYT_RS09230 - NADPH:quinone reductase, at 2,090,010 to 2,091,032 _RS09230 BPHYT_RS09235: BPHYT_RS09235 - hypothetical protein, at 2,091,386 to 2,091,943 _RS09235 BPHYT_RS09240: BPHYT_RS09240 - ABC transporter permease, at 2,091,982 to 2,092,788 _RS09240 BPHYT_RS09245: BPHYT_RS09245 - ABC transporter ATP-binding protein, at 2,092,785 to 2,093,513 _RS09245
Group Condition BPHYT_RS09225 BPHYT_RS09230 BPHYT_RS09235 BPHYT_RS09240 BPHYT_RS09245
supernatant control Vogels_fungal_media; pH 5.5 -0.8 -1.1 +0.1 +0.1 -1.6
phage 36hr PHYTO6 MOI 1 -0.2 -1.1 -0.2 -0.4 -0.5
phage 24hr PHYTO1 MOI 10 -0.7 -1.2 -0.4 -0.1 -0.0
phage 24hr PHYTO11 MOI 0.1 -1.7 -0.0 -0.3 -0.6 +0.8
phage 36hr PHYTO8 MOI 10 -0.3 -0.7 +0.4 -0.4 -0.8
phage 24hr PHYTO6 MOI 1 -0.9 -0.2 -0.1 -0.7 +0.2
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) -1.0 -0.3 -0.6 -0.1 +0.5
stress Bacitracin 0.25 mg/ml -0.2 +0.4 -0.5 -0.2 -0.9
phage 24hr PHYTO2 MOI 0.1 -0.6 -0.5 -0.2 -0.4 +0.4
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.5 -0.1 -0.9 -0.2 +0.4
phage 24hr PHYTO9 MOI 10 -0.1 -1.1 -0.4 -0.2 +0.6
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.6 -0.6 +0.3 -0.3 +0.4
carbon source Protocatechuic Acid (C) -0.7 -0.6 +0.1 -0.2 +0.6
phage 36hr PHYTO6 MOI 0.1 -1.0 -0.0 -0.2 -0.3 +0.9
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.8 -0.3 -0.2 -0.5 +1.2
phage 36hr PHYTO11 MOI 0.1 -0.8 -0.7 +0.2 +0.1 +0.6
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.8 -0.0 -0.5 -0.2 -0.7
phage 24hr PHYTO11 MOI 1 -0.6 -0.6 -0.1 +0.0 +0.8
phage 36hr PHYTO2 MOI 1 -0.6 -0.8 +0.5 -0.1 +0.6
phage 24hr PHYTO6 MOI 10 -0.4 +0.1 +0.9 -0.1 -0.7
phage 24hr PHYTO2 MOI 1 -1.2 -0.3 +0.4 +0.2 +0.9
in planta Plant=Brachypodium_distachyon ; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.4 +0.2 -0.9 +0.1 +0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.2 +0.2 +1.5 -0.3 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.5 +1.4 -0.0 -0.2 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.1 -0.2 -0.4 +0.4 +1.2
nitrogen source L-Alanine (N) -0.4 -0.4 +0.6 +0.5 +0.8
nitrogen source L-Glutamine (N) +0.3 -0.4 +0.5 +0.4 +0.5
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days +0.6 +0.5 +0.7 +0.1 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.1 +0.6 +0.4 +0.7 -0.3
nitrogen source L-Proline (N) +0.4 -0.3 +0.9 +0.2 +1.0
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