Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS09040 and BPHYT_RS09045 are separated by 223 nucleotidesBPHYT_RS09045 and BPHYT_RS09050 are separated by 234 nucleotidesBPHYT_RS09050 and BPHYT_RS09055 are separated by 781 nucleotidesBPHYT_RS09055 and BPHYT_RS09060 are separated by 67 nucleotides BPHYT_RS09040: BPHYT_RS09040 - MerR family transcriptional regulator, at 2,045,859 to 2,046,410 _RS09040 BPHYT_RS09045: BPHYT_RS09045 - transcription termination factor Rho, at 2,046,634 to 2,047,902 _RS09045 BPHYT_RS09050: BPHYT_RS09050 - thioredoxin, at 2,048,137 to 2,048,463 _RS09050 BPHYT_RS09055: BPHYT_RS09055 - DNA polymerase III subunit gamma/tau, at 2,049,245 to 2,051,926 _RS09055 BPHYT_RS09060: BPHYT_RS09060 - hypothetical protein, at 2,051,994 to 2,052,320 _RS09060
Group Condition BPHYT_RS09040 BPHYT_RS09045 BPHYT_RS09050 BPHYT_RS09055 BPHYT_RS09060
phage 36hr PHYTO2 MOI 1 -1.1 N.D. N.D. N.D. N.D.
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent -1.1 N.D. N.D. N.D. N.D.
phage 24hr PHYTO6 MOI 10 -1.1 N.D. N.D. N.D. N.D.
phage 24hr PHYTO2 MOI 0.1 -1.0 N.D. N.D. N.D. N.D.
phage 36hr PHYTO9 MOI 1 -1.0 N.D. N.D. N.D. N.D.
phage 36hr PHYTO1 MOI 1 -1.0 N.D. N.D. N.D. N.D.
phage 36hr PHYTO1 MOI 10 -0.9 N.D. N.D. N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB -0.9 N.D. N.D. N.D. N.D.
carbon source Protocatechuic Acid (C) -0.8 N.D. N.D. N.D. N.D.
lb_dap plate control LB_DAP agar plate, outgrowth in LB -0.8 N.D. N.D. N.D. N.D.
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -0.8 N.D. N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.7 N.D. N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.7 N.D. N.D. N.D. N.D.
phage P. phyto corn MOI 1 -0.7 N.D. N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.7 N.D. N.D. N.D. N.D.
nophagecontrol 36hr Phyto Lib only -0.7 N.D. N.D. N.D. N.D.
phage P. phyto PHYTO2 MOI 1 -0.7 N.D. N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.6 N.D. N.D. N.D. N.D.
motility outer cut, LB soft agar motility assay +0.6 N.D. N.D. N.D. N.D.
temperature LB, 37C +0.7 N.D. N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.7 N.D. N.D. N.D. N.D.
phage 36hr PHYTO7 MOI 1 +0.7 N.D. N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.7 N.D. N.D. N.D. N.D.
motility inner cut, LB soft agar motility assay +0.7 N.D. N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.9 N.D. N.D. N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X +0.9 N.D. N.D. N.D. N.D.
carbon source Phenylacetic 5 mM (C) +1.0 N.D. N.D. N.D. N.D.
vitamin No vitamins t1 +1.0 N.D. N.D. N.D. N.D.
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) +1.0 N.D. N.D. N.D. N.D.
phage 24hr PHYTO3 MOI 10 +1.2 N.D. N.D. N.D. N.D.
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