Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS09005 and BPHYT_RS09010 overlap by 49 nucleotidesBPHYT_RS09010 and BPHYT_RS09015 are separated by 330 nucleotidesBPHYT_RS09015 and BPHYT_RS09020 are separated by 2 nucleotidesBPHYT_RS09020 and BPHYT_RS09025 are separated by 294 nucleotides BPHYT_RS09005: BPHYT_RS09005 - MerR family transcriptional regulator, at 2,038,283 to 2,038,777 _RS09005 BPHYT_RS09010: BPHYT_RS09010 - 4-carboxymuconolactone decarboxylase, at 2,038,729 to 2,039,187 _RS09010 BPHYT_RS09015: BPHYT_RS09015 - multidrug transporter MatE, at 2,039,518 to 2,040,897 _RS09015 BPHYT_RS09020: BPHYT_RS09020 - UDP phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase, at 2,040,900 to 2,042,657 _RS09020 BPHYT_RS09025: BPHYT_RS09025 - 50S ribosomal protein L31 type B, at 2,042,952 to 2,043,218 _RS09025
Group Condition BPHYT_RS09005 BPHYT_RS09010 BPHYT_RS09015 BPHYT_RS09020 BPHYT_RS09025
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB -0.6 -0.5 -0.2 N.D. N.D.
phage 24hr PHYTO9 MOI 10 -0.1 -1.0 -0.1 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.6 -0.5 -0.2 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.6 -0.1 -0.4 N.D. N.D.
carbon source Suberic (C) +0.0 -1.0 -0.0 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.5 -0.5 -0.0 N.D. N.D.
phage 24hr PHYTO4 MOI 10 -0.4 -0.3 -0.2 N.D. N.D.
phage 36hr PHYTO10 MOI 10 -0.1 -0.7 -0.1 N.D. N.D.
nophagecontrol 24hr Phyto Lib Only +0.1 -0.9 -0.1 N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.2 +0.0 -0.7 N.D. N.D.
supernatant control Vogels_fungal_media; pH 5.5 -0.4 -0.5 +0.1 N.D. N.D.
nitrogen source L-Lysine (N) +0.2 -0.8 -0.1 N.D. N.D.
carbon source Phenylacetic 5 mM (C) +0.2 -0.6 -0.2 N.D. N.D.
nitrogen source L-Phenylalanine (N) -0.1 -0.7 +0.2 N.D. N.D.
nophagecontrol 36hr Phyto Lib only +0.3 -0.7 +0.1 N.D. N.D.
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -0.5 +0.4 -0.3 N.D. N.D.
phage 36hr PHYTO7 MOI 1 +0.5 -0.3 -0.3 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 -0.5 +0.2 N.D. N.D.
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 +0.1 +0.5 -0.4 N.D. N.D.
phage 36hr PHYTO4 MOI 1 -0.2 +0.8 -0.3 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.3 +0.7 -0.1 N.D. N.D.
stress Paraquat dichloride 0.064 mg/ml +0.5 -0.4 +0.2 N.D. N.D.
vitamin With vitamins t1 -0.3 +0.2 +0.4 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.4 -0.3 +0.3 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.4 +0.3 -0.3 N.D. N.D.
no phage control No phage control -0.1 +0.8 -0.1 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 +0.7 +0.0 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.1 +0.8 +0.2 N.D. N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.3 +0.7 +0.3 N.D. N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.4 +0.7 +0.3 N.D. N.D.
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