Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS08720 and BPHYT_RS08725 are separated by 60 nucleotidesBPHYT_RS08725 and BPHYT_RS08730 are separated by 206 nucleotidesBPHYT_RS08730 and BPHYT_RS08735 are separated by 170 nucleotidesBPHYT_RS08735 and BPHYT_RS08740 are separated by 138 nucleotides BPHYT_RS08720: BPHYT_RS08720 - membrane protein, at 1,979,774 to 1,980,400 _RS08720 BPHYT_RS08725: BPHYT_RS08725 - hypothetical protein, at 1,980,461 to 1,980,706 _RS08725 BPHYT_RS08730: BPHYT_RS08730 - hypothetical protein, at 1,980,913 to 1,981,125 _RS08730 BPHYT_RS08735: BPHYT_RS08735 - aldo-keto reductase, at 1,981,296 to 1,982,348 _RS08735 BPHYT_RS08740: BPHYT_RS08740 - membrane protein, at 1,982,487 to 1,983,770 _RS08740
Group Condition BPHYT_RS08720 BPHYT_RS08725 BPHYT_RS08730 BPHYT_RS08735 BPHYT_RS08740
nitrogen source L-Lysine (N) -0.5 N.D. -0.5 -0.0 -0.4
phage 36hr PHYTO11 MOI 0.1 -0.6 N.D. -0.2 +0.3 -0.3
nutrient L-Serine +0.3 N.D. -0.4 -0.1 -0.4
carbon source D-Glucosamine Hydrochloride (C) -0.5 N.D. -0.7 +0.7 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.3 N.D. -0.3 -0.1 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.1 N.D. +0.3 -0.3 -0.4
phage 24hr PHYTO6 MOI 1 -0.0 N.D. -0.6 +0.5 -0.2
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 -0.4 N.D. -0.6 +0.3 +0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days -0.4 N.D. -0.3 -0.0 +0.5
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) -0.3 N.D. -0.2 +0.6 -0.2
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent +0.4 N.D. -0.1 -0.7 +0.1
phage 36hr PHYTO10 MOI 1 +0.2 N.D. -0.2 +0.4 -0.6
phage 36hr PHYTO1 MOI 1 +0.4 N.D. -0.3 +0.3 -0.3
phage 36hr PHYTO10 MOI 10 +0.4 N.D. -0.1 +0.3 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.0 N.D. +0.7 +0.3 -0.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days -0.4 N.D. -0.0 +0.1 +0.7
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.6 N.D. -0.3 +0.4 -0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB +0.4 N.D. +0.5 -0.1 -0.4
nophagecontrol 36hr Phyto Lib Only +0.6 N.D. +0.3 +0.1 -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 N.D. +0.5 +0.2 -0.5
carbon source D,L-Lactate (C) +0.2 N.D. +0.2 -0.3 +0.4
phage 24hr PHYTO11 MOI 0.1 +0.3 N.D. +0.0 +0.5 -0.3
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.6 N.D. +0.4 -0.4 -0.0
phage 24hr PHYTO6 MOI 0.1 +0.1 N.D. +0.2 +0.5 -0.3
nitrogen source L-Glutamic (N) +0.4 N.D. -0.4 +0.5 +0.1
phage 24hr PHYTO2 MOI 1 +0.4 N.D. -0.2 +0.7 -0.2
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days +0.3 N.D. +0.6 +0.3 +0.0
phage 36hr PHYTO6 MOI 1 +0.5 N.D. -0.0 +0.5 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.5 N.D. +0.4 +0.3 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.4 N.D. +0.4 +0.6 +0.2
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