Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS08515 and BPHYT_RS08520 are separated by 324 nucleotidesBPHYT_RS08520 and BPHYT_RS08525 are separated by 440 nucleotidesBPHYT_RS08525 and BPHYT_RS08530 are separated by 103 nucleotidesBPHYT_RS08530 and BPHYT_RS08535 overlap by 26 nucleotides BPHYT_RS08515: BPHYT_RS08515 - acyl carrier protein, at 1,948,868 to 1,949,119 _RS08515 BPHYT_RS08520: BPHYT_RS08520 - GNAT family acetyltransferase, at 1,949,444 to 1,949,977 _RS08520 BPHYT_RS08525: BPHYT_RS08525 - SAM-dependent methyltransferase, at 1,950,418 to 1,951,251 _RS08525 BPHYT_RS08530: BPHYT_RS08530 - mannose-1-phosphate guanylyltransferase (GDP), at 1,951,355 to 1,952,572 _RS08530 BPHYT_RS08535: BPHYT_RS08535 - flavin reductase, at 1,952,547 to 1,953,137 _RS08535
Group Condition BPHYT_RS08515 BPHYT_RS08520 BPHYT_RS08525 BPHYT_RS08530 BPHYT_RS08535
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. N.D. -4.5 N.D. N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. N.D. -3.2 N.D. N.D.
carbon source 2-Deoxy-D-ribonic lithium salt (C) N.D. N.D. -3.2 N.D. N.D.
phage P. phyto PHYTO2 MOI 1 N.D. N.D. -3.0 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. -3.0 N.D. N.D.
phage 24hr PHYTO3 MOI 10 N.D. N.D. -2.9 N.D. N.D.
nophagecontrol 36hr Phyto Lib Only N.D. N.D. -2.8 N.D. N.D.
phage 36hr PHYTO6 MOI 0.1 N.D. N.D. -2.8 N.D. N.D.
phage 24hr PHYTO2 MOI 0.1 N.D. N.D. -2.7 N.D. N.D.
phage 24hr PHYTO10 MOI 10 N.D. N.D. -2.7 N.D. N.D.
phage 24hr PHYTO11 MOI 0.1 N.D. N.D. -2.6 N.D. N.D.
phage 36hr PHYTO8 MOI 10 N.D. N.D. -2.6 N.D. N.D.
phage 36hr PHYTO7 MOI 1 N.D. N.D. -2.5 N.D. N.D.
phage 36hr PHYTO4 MOI 1 N.D. N.D. -2.4 N.D. N.D.
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 N.D. N.D. -2.4 N.D. N.D.
vitamin With vitamins t2 N.D. N.D. -2.2 N.D. N.D.
no phage control No phage control N.D. N.D. -2.1 N.D. N.D.
phage 24hr PHYTO4 MOI 10 N.D. N.D. -2.1 N.D. N.D.
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) N.D. N.D. -1.9 N.D. N.D.
stress Paraquat dichloride 0.032 mg/ml N.D. N.D. -1.7 N.D. N.D.
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent N.D. N.D. -1.7 N.D. N.D.
carbon source Suberic (C) N.D. N.D. -1.7 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. -1.6 N.D. N.D.
carbon source L-Arabinose (C) N.D. N.D. -1.6 N.D. N.D.
nitrogen source Ammonium chloride (N) N.D. N.D. -1.6 N.D. N.D.
carbon source D-Glucose (C) N.D. N.D. +1.6 N.D. N.D.
nitrogen source L-Isoleucine (N) N.D. N.D. +1.7 N.D. N.D.
nitrogen source L-Glutamine (N) N.D. N.D. +1.7 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. +1.7 N.D. N.D.
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. +1.8 N.D. N.D.
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