Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS08460 and BPHYT_RS08465 are separated by 81 nucleotidesBPHYT_RS08465 and BPHYT_RS08470 are separated by 472 nucleotidesBPHYT_RS08470 and BPHYT_RS08475 are separated by 197 nucleotidesBPHYT_RS08475 and BPHYT_RS08480 overlap by 4 nucleotides BPHYT_RS08460: BPHYT_RS08460 - 23S rRNA (guanosine-2'-O-)-methyltransferase, at 1,935,640 to 1,936,383 _RS08460 BPHYT_RS08465: BPHYT_RS08465 - ribose-5-phosphate isomerase A, at 1,936,465 to 1,937,160 _RS08465 BPHYT_RS08470: BPHYT_RS08470 - glycosyl transferase, at 1,937,633 to 1,938,829 _RS08470 BPHYT_RS08475: BPHYT_RS08475 - hypothetical protein, at 1,939,027 to 1,939,326 _RS08475 BPHYT_RS08480: BPHYT_RS08480 - transcriptional regulator, at 1,939,323 to 1,939,745 _RS08480
Group Condition BPHYT_RS08460 BPHYT_RS08465 BPHYT_RS08470 BPHYT_RS08475 BPHYT_RS08480
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) N.D. N.D. -1.1 -0.7 N.D.
nitrogen source Uridine (N) N.D. N.D. -0.8 -0.5 N.D.
carbon source D-Glucose (C) N.D. N.D. -0.6 -0.5 N.D.
nitrogen source L-Serine (N) N.D. N.D. -0.3 -0.9 N.D.
carbon source 2-Deoxy-D-Ribose (C) N.D. N.D. -0.5 -0.6 N.D.
r2a control R2A control N.D. N.D. -0.5 -0.5 N.D.
carbon source 2-Deoxy-D-Ribose (C) N.D. N.D. -0.5 -0.5 N.D.
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days N.D. N.D. -0.3 -0.6 N.D.
carbon source Sebacic (C) N.D. N.D. -0.0 -0.9 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. N.D. -0.6 +0.3 N.D.
supernatant control Vogels_fungal_media; pH 5.5 N.D. N.D. +0.4 -0.6 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. N.D. -0.7 +0.5 N.D.
carbon source Tween 20 (C) N.D. N.D. +0.5 -0.7 N.D.
phage 36hr PHYTO4 MOI 1 N.D. N.D. +0.6 -0.7 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. N.D. -0.6 +0.5 N.D.
phage 36hr PHYTO11 MOI 1 N.D. N.D. +0.7 -0.7 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days N.D. N.D. +0.6 -0.3 N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. -0.3 +0.6 N.D.
phage 24hr PHYTO1 MOI 10 N.D. N.D. +0.7 -0.3 N.D.
phage 36hr PHYTO7 MOI 0.1 N.D. N.D. +0.9 -0.3 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. -0.2 +1.1 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. +0.2 +0.7 N.D.
phage 36hr PHYTO10 MOI 10 N.D. N.D. +0.5 +0.4 N.D.
phage 36hr PHYTO7 MOI 1 N.D. N.D. +0.5 +0.4 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. -0.1 +1.1 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. +0.4 +0.6 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. +0.1 +1.1 N.D.
carbon source Malonic (C) N.D. N.D. +0.7 +0.5 N.D.
phage 24hr PHYTO3 MOI 10 N.D. N.D. +0.9 +0.5 N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. +0.3 +1.3 N.D.
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