Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS08370 and BPHYT_RS08375 are separated by 42 nucleotidesBPHYT_RS08375 and BPHYT_RS08380 are separated by 486 nucleotidesBPHYT_RS08380 and BPHYT_RS08385 are separated by 137 nucleotidesBPHYT_RS08385 and BPHYT_RS08390 overlap by 4 nucleotides BPHYT_RS08370: BPHYT_RS08370 - glyoxalase, at 1,917,751 to 1,918,362 _RS08370 BPHYT_RS08375: BPHYT_RS08375 - enoyl-CoA hydratase, at 1,918,405 to 1,919,268 _RS08375 BPHYT_RS08380: BPHYT_RS08380 - multidrug MFS transporter, at 1,919,755 to 1,921,080 _RS08380 BPHYT_RS08385: BPHYT_RS08385 - secretion protein, at 1,921,218 to 1,922,756 _RS08385 BPHYT_RS08390: BPHYT_RS08390 - DSBA oxidoreductase, at 1,922,753 to 1,923,829 _RS08390
Group Condition BPHYT_RS08370 BPHYT_RS08375 BPHYT_RS08380 BPHYT_RS08385 BPHYT_RS08390
phage 24hr PHYTO9 MOI 10 N.D. N.D. -0.3 -3.9 N.D.
phage 36hr PHYTO9 MOI 0.1 N.D. N.D. +0.2 -4.2 N.D.
phage 36hr PHYTO8 MOI 10 N.D. N.D. +0.1 -4.0 N.D.
phage 36hr PHYTO6 MOI 0.1 N.D. N.D. +0.2 -4.1 N.D.
phage 24hr PHYTO MOI 0.1 N.D. N.D. -0.1 -2.5 N.D.
phage 36hr PHYTO4 MOI 1 N.D. N.D. -0.0 -2.3 N.D.
phage 36hr PHYTO10 MOI 10 N.D. N.D. +0.3 -2.4 N.D.
phage 36hr PHYTO2 MOI 0.1 N.D. N.D. -0.1 -1.9 N.D.
carbon source Sebacic (C) N.D. N.D. -0.1 -1.9 N.D.
phage 24hr PHYTO6 MOI 0.1 N.D. N.D. -0.1 -1.8 N.D.
nophagecontrol 24hr Phyto Lib Only N.D. N.D. -0.5 -1.4 N.D.
phage 24hr PHYTO2 MOI 0.1 N.D. N.D. +0.1 -1.9 N.D.
phage 24hr PHYTO2 MOI 0.1 N.D. N.D. +0.0 -1.8 N.D.
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 N.D. N.D. -0.6 -1.1 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. N.D. -0.3 -1.3 N.D.
phage 36hr PHYTO10 MOI 1 N.D. N.D. +0.1 -1.7 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB N.D. N.D. +0.5 -1.7 N.D.
carbon source 2-Deoxy-D-ribonic lithium salt (C) N.D. N.D. +0.5 -1.7 N.D.
nophagecontrol 36hr Phyto Lib only N.D. N.D. +0.5 -1.6 N.D.
phage 36hr PHYTO MOI 0.1 N.D. N.D. +0.5 -1.5 N.D.
phage 36hr PHYTO10 MOI 10 N.D. N.D. +0.4 -1.2 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. +0.7 -1.0 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. +1.5 -1.6 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. N.D. -0.4 +1.5 N.D.
vitamin With vitamins t1 N.D. N.D. -0.1 +1.5 N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. +0.5 +1.3 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X N.D. N.D. +0.8 +1.1 N.D.
vitamin No vitamins t1 N.D. N.D. +0.3 +1.7 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X N.D. N.D. +0.9 +1.4 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. N.D. +3.6 +0.4 N.D.
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