Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS08170 and BPHYT_RS08175 are separated by 42 nucleotidesBPHYT_RS08175 and BPHYT_RS08180 are separated by 132 nucleotidesBPHYT_RS08180 and BPHYT_RS08185 are separated by 3 nucleotidesBPHYT_RS08185 and BPHYT_RS08190 overlap by 8 nucleotides BPHYT_RS08170: BPHYT_RS08170 - pilus assembly protein CpaB, at 1,867,754 to 1,868,704 _RS08170 BPHYT_RS08175: BPHYT_RS08175 - membrane protein, at 1,868,747 to 1,870,153 _RS08175 BPHYT_RS08180: BPHYT_RS08180 - fimbrial protein, at 1,870,286 to 1,871,527 _RS08180 BPHYT_RS08185: BPHYT_RS08185 - pilus assembly protein CpaF, at 1,871,531 to 1,872,940 _RS08185 BPHYT_RS08190: BPHYT_RS08190 - type II secretion system protein, at 1,872,933 to 1,873,961 _RS08190
Group Condition BPHYT_RS08170 BPHYT_RS08175 BPHYT_RS08180 BPHYT_RS08185 BPHYT_RS08190
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB -0.3 N.D. +0.0 -0.3 -2.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.2 N.D. +0.2 -0.9 -1.4
phage 24hr PHYTO9 MOI 10 -0.4 N.D. -0.1 -0.5 -1.1
carbon source D-Lactate (C) -0.3 N.D. -0.7 -0.8 -0.2
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days +0.1 N.D. -0.3 -0.1 -1.7
phage 24hr PHYTO10 MOI 10 -0.8 N.D. +0.1 -0.1 -1.2
phage 24hr PHYTO11 MOI 1 -1.2 N.D. -0.7 -0.4 +0.3
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days +0.3 N.D. +0.3 -0.2 -1.9
carbon source D-Glucose 20 mM (C) -0.6 N.D. -0.7 +0.6 -0.4
carbon source 2-Deoxy-D-ribonic lithium salt (C) +1.0 N.D. -0.3 -0.2 -1.5
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 -1.4 N.D. +0.4 +0.6 -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.6 N.D. -0.8 +0.8 -0.3
nitrogen source Putrescine (N) +0.8 N.D. -0.2 -0.2 -1.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.4 N.D. -0.3 +1.0 -1.0
phage 24hr PHYTO MOI 0.1 +0.9 N.D. +0.3 -0.5 -0.5
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.8 N.D. -0.1 -0.1 +1.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days -0.8 N.D. +0.1 -0.4 +1.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days -1.2 N.D. +0.2 +0.6 +1.4
carbon source L-Fucose (C) -0.5 N.D. +0.4 +0.4 +0.9
carbon source adipate (C) +0.4 N.D. -0.5 +0.2 +1.1
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.8 N.D. -0.5 +0.3 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.1 N.D. +0.1 +1.8 -0.5
motility outer cut, LB soft agar motility assay +0.7 N.D. -0.2 -0.2 +1.3
carbon source 2-Deoxy-D-Ribose (C) +0.5 N.D. -0.2 +0.6 +0.8
agar plate interaction control Parafilmed volatile agar plate with no fungus +1.5 N.D. -0.4 -0.0 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.2 N.D. +0.7 +1.4 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.8 N.D. -0.1 +0.2 +1.2
carbon source Azelaic (C) +0.5 N.D. +0.3 +0.2 +1.1
carbon source D-Xylose (C) +0.4 N.D. +0.2 +0.5 +1.1
carbon source Malonic (C) -0.4 N.D. +0.3 +0.5 +2.6
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