Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS07625 and BPHYT_RS07630 are separated by 88 nucleotidesBPHYT_RS07630 and BPHYT_RS07635 are separated by 316 nucleotidesBPHYT_RS07635 and BPHYT_RS07640 are separated by 56 nucleotidesBPHYT_RS07640 and BPHYT_RS07645 are separated by 128 nucleotides BPHYT_RS07625: BPHYT_RS07625 - alpha/beta hydrolase, at 1,735,732 to 1,736,847 _RS07625 BPHYT_RS07630: BPHYT_RS07630 - alkane 1-monooxygenase, at 1,736,936 to 1,737,931 _RS07630 BPHYT_RS07635: BPHYT_RS07635 - alkanesulfonate monooxygenase, at 1,738,248 to 1,739,327 _RS07635 BPHYT_RS07640: BPHYT_RS07640 - hypothetical protein, at 1,739,384 to 1,740,163 _RS07640 BPHYT_RS07645: BPHYT_RS07645 - cysteine dioxygenase, at 1,740,292 to 1,740,900 _RS07645
Group Condition BPHYT_RS07625 BPHYT_RS07630 BPHYT_RS07635 BPHYT_RS07640 BPHYT_RS07645
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 -0.5 -0.3 -0.5 -0.3 -1.0
carbon source Phenylacetic 5 mM (C) -0.2 -0.5 -1.1 +0.1 -0.8
phage P. phyto PHYTO2 MOI 1 -0.4 -0.3 -0.5 -0.7 -0.1
nitrogen source L-Serine (N) +0.1 +0.2 -1.1 -0.6 -0.4
nitrogen source NAG (N) +0.4 -0.4 -1.4 -0.3 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.0 +0.0 -1.4 +0.4 -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.5 -0.3 +0.6 -1.0 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.0 -0.4 +0.8 -0.9 -0.5
r2a control R2A control +0.2 -0.4 +0.3 -0.5 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.0 +0.7 -0.4 -1.1 +0.2
nitrogen source L-Glutamic (N) +0.2 +0.0 -1.1 +0.5 -0.1
phage 24hr PHYTO7 MOI 1 +0.3 -0.6 +0.7 -0.6 +0.1
nophagecontrol 36hr Phyto Lib only -0.2 -0.7 +0.4 +0.6 +0.1
phage 36hr PHYTO2 MOI 1 -0.1 -0.8 +0.6 +0.0 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.0 -0.2 +0.9 -0.4 +0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.3 -0.1 -0.6 +1.1 +0.5
phage 36hr PHYTO3 MOI 1 -0.3 -0.4 +0.7 +0.4 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.2 +0.2 -0.4 +1.0 +0.2
phage 36hr PHYTO6 MOI 10 +0.2 -0.3 +0.9 -0.3 +0.3
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +0.8 +0.3 +0.1 +0.3 -0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.1 +0.3 -0.4 +1.1 +0.0
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 -0.2 -0.1 +1.0 +0.1 +0.5
phage P. phyto PHYTO2 MOI 1 +0.2 +1.1 -1.0 +1.0 +0.2
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) +0.2 +0.8 +0.2 -0.4 +0.8
in planta Plant=Zea_mays; PlantTreatment=None; sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_R2A_with_Tween_and_Cycloheximide; Time=15_days +0.0 +0.2 +1.2 +0.4 +0.2
phage 24hr PHYTO1 MOI 10 +0.2 +0.2 +0.7 +0.2 +0.6
phage 36hr PHYTO10 MOI 10 +0.1 +0.3 +0.5 +0.8 +0.3
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days +0.2 +0.4 +0.8 +0.6 +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 +0.3 +1.6 +0.3 +0.0
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days +0.3 +0.5 +0.8 +0.7 +0.4
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BPHYT_RS07630
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