Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS07480 and BPHYT_RS07485 are separated by 113 nucleotidesBPHYT_RS07485 and BPHYT_RS07490 are separated by 213 nucleotidesBPHYT_RS07490 and BPHYT_RS07495 are separated by 22 nucleotidesBPHYT_RS07495 and BPHYT_RS07500 are separated by 117 nucleotides BPHYT_RS07480: BPHYT_RS07480 - FAD-dependent oxidoreductase, at 1,700,503 to 1,701,894 _RS07480 BPHYT_RS07485: BPHYT_RS07485 - cupin, at 1,702,008 to 1,702,379 _RS07485 BPHYT_RS07490: BPHYT_RS07490 - aldehyde dehydrogenase, at 1,702,593 to 1,704,029 _RS07490 BPHYT_RS07495: BPHYT_RS07495 - LysR family transcriptional regulator, at 1,704,052 to 1,705,002 _RS07495 BPHYT_RS07500: BPHYT_RS07500 - amidohydrolase, at 1,705,120 to 1,706,292 _RS07500
Group Condition BPHYT_RS07480 BPHYT_RS07485 BPHYT_RS07490 BPHYT_RS07495 BPHYT_RS07500
phage 24hr PHYTO10 MOI 1 -3.8 N.D. +0.3 N.D. -0.9
nophagecontrol 36hr Phyto Lib only -3.7 N.D. -0.1 N.D. -0.3
phage 36hr PHYTO8 MOI 0.1 -3.7 N.D. +0.3 N.D. -0.5
phage 36hr PHYTO1 MOI 10 -2.3 N.D. -0.5 N.D. -0.6
phage 24hr PHYTO2 MOI 0.1 -3.8 N.D. +0.5 N.D. -0.1
phage 24hr PHYTO4 MOI 10 -3.1 N.D. +0.2 N.D. -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus -2.7 N.D. -0.3 N.D. -0.2
phage 36hr PHYTO3 MOI 1 -1.6 N.D. +0.4 N.D. -1.7
phage 36hr PHYTO7 MOI 1 -2.0 N.D. +0.0 N.D. -1.0
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -1.4 N.D. -0.7 N.D. -0.7
phage 24hr PHYTO MOI 0.1 -1.4 N.D. +0.1 N.D. -1.4
phage 36hr PHYTO4 MOI 1 -1.5 N.D. -0.1 N.D. -1.1
phage 36hr PHYTO6 MOI 0.1 -2.2 N.D. +0.1 N.D. -0.5
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X -2.1 N.D. +0.4 N.D. -0.7
carbon source Dodecandioic (C) -2.5 N.D. +0.2 N.D. -0.2
nophagecontrol 36hr Phyto Lib Only -2.2 N.D. +0.2 N.D. -0.4
carbon source 2-Deoxy-D-ribonic lithium salt (C) -2.7 N.D. +0.6 N.D. -0.1
phage 24hr PHYTO10 MOI 10 -2.3 N.D. +0.0 N.D. +0.2
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 -1.4 N.D. +0.2 N.D. -0.8
carbon source D-Glucose 20 mM (C) -2.2 N.D. -0.0 N.D. +0.3
phage 36hr PHYTO7 MOI 0.1 -2.3 N.D. +0.7 N.D. +0.1
phage 36hr PHYTO6 MOI 10 -0.8 N.D. +0.6 N.D. -1.1
phage 36hr PHYTO2 MOI 1 -1.6 N.D. +0.5 N.D. -0.3
phage 24hr PHYTO6 MOI 1 -1.1 N.D. +0.6 N.D. -0.6
phage 24hr PHYTO3 MOI 10 -1.4 N.D. +1.0 N.D. -0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days +1.7 N.D. -0.5 N.D. -0.0
agar plate interaction control Parafilmed volatile agar plate with no fungus +1.6 N.D. -0.7 N.D. +0.4
carbon source Malonic (C) +1.8 N.D. +0.3 N.D. -0.8
phage 24hr PHYTO6 MOI 10 +1.9 N.D. +0.5 N.D. -0.4
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +2.9 N.D. +0.6 N.D. -0.9
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