Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS07475 and BPHYT_RS07480 are separated by 194 nucleotidesBPHYT_RS07480 and BPHYT_RS07485 are separated by 113 nucleotidesBPHYT_RS07485 and BPHYT_RS07490 are separated by 213 nucleotidesBPHYT_RS07490 and BPHYT_RS07495 are separated by 22 nucleotides BPHYT_RS07475: BPHYT_RS07475 - nitrate reductase, at 1,698,848 to 1,700,308 _RS07475 BPHYT_RS07480: BPHYT_RS07480 - FAD-dependent oxidoreductase, at 1,700,503 to 1,701,894 _RS07480 BPHYT_RS07485: BPHYT_RS07485 - cupin, at 1,702,008 to 1,702,379 _RS07485 BPHYT_RS07490: BPHYT_RS07490 - aldehyde dehydrogenase, at 1,702,593 to 1,704,029 _RS07490 BPHYT_RS07495: BPHYT_RS07495 - LysR family transcriptional regulator, at 1,704,052 to 1,705,002 _RS07495
Group Condition BPHYT_RS07475 BPHYT_RS07480 BPHYT_RS07485 BPHYT_RS07490 BPHYT_RS07495
phage 24hr PHYTO10 MOI 10 -2.7 -2.3 N.D. +0.0 N.D.
nophagecontrol 36hr Phyto Lib only -0.9 -3.7 N.D. -0.1 N.D.
phage 24hr PHYTO10 MOI 1 -0.9 -3.8 N.D. +0.3 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.4 -2.7 N.D. -0.3 N.D.
phage 24hr PHYTO2 MOI 0.1 -0.0 -3.8 N.D. +0.5 N.D.
phage 36hr PHYTO6 MOI 0.1 -1.1 -2.2 N.D. +0.1 N.D.
phage 36hr PHYTO1 MOI 10 -0.3 -2.3 N.D. -0.5 N.D.
phage 36hr PHYTO11 MOI 0.1 -2.0 -1.0 N.D. -0.0 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -2.0 -0.9 N.D. -0.1 N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.8 -1.4 N.D. -0.7 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.2 -1.7 N.D. +0.0 N.D.
phage 36hr PHYTO2 MOI 1 -2.5 -0.4 N.D. +0.1 N.D.
phage 24hr PHYTO4 MOI 10 +0.2 -3.1 N.D. +0.2 N.D.
phage 36hr PHYTO6 MOI 10 -2.5 -0.8 N.D. +0.6 N.D.
phage 36hr PHYTO8 MOI 0.1 +0.7 -3.7 N.D. +0.3 N.D.
carbon source 2-Deoxy-D-ribonic lithium salt (C) -0.4 -2.7 N.D. +0.6 N.D.
phage 24hr PHYTO6 MOI 1 -2.0 -1.1 N.D. +0.6 N.D.
nophagecontrol 36hr Phyto Lib Only -0.3 -2.2 N.D. +0.2 N.D.
phage 24hr PHYTO3 MOI 10 -1.6 -1.2 N.D. +0.8 N.D.
phage 36hr PHYTO2 MOI 1 -0.9 -1.6 N.D. +0.5 N.D.
phage 36hr PHYTO MOI 0.1 -2.8 +0.1 N.D. +0.8 N.D.
phage 36hr PHYTO10 MOI 10 -1.8 -0.5 N.D. +0.5 N.D.
phage 24hr PHYTO1 MOI 10 -1.5 -0.7 N.D. +0.6 N.D.
phage 24hr PHYTO3 MOI 10 -0.9 -1.4 N.D. +1.0 N.D.
carbon source D-Glucose 20 mM (C) +1.0 -2.2 N.D. -0.0 N.D.
phage 36hr PHYTO7 MOI 0.1 +0.4 -2.3 N.D. +0.7 N.D.
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X +0.6 -2.1 N.D. +0.4 N.D.
phage 36hr PHYTO6 MOI 1 -1.1 -0.7 N.D. +1.0 N.D.
phage 24hr PHYTO6 MOI 10 -0.4 +1.9 N.D. +0.5 N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 +2.9 N.D. +0.6 N.D.
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