Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS07310 and BPHYT_RS07315 are separated by 205 nucleotidesBPHYT_RS07315 and BPHYT_RS07320 are separated by 89 nucleotidesBPHYT_RS07320 and BPHYT_RS07325 are separated by 50 nucleotidesBPHYT_RS07325 and BPHYT_RS07330 are separated by 276 nucleotides BPHYT_RS07310: BPHYT_RS07310 - glyoxalase, at 1,658,975 to 1,659,403 _RS07310 BPHYT_RS07315: BPHYT_RS07315 - lipase, at 1,659,609 to 1,660,565 _RS07315 BPHYT_RS07320: BPHYT_RS07320 - dihydrodipicolinate synthetase, at 1,660,655 to 1,661,563 _RS07320 BPHYT_RS07325: BPHYT_RS07325 - GntR family transcriptional regulator, at 1,661,614 to 1,662,273 _RS07325 BPHYT_RS07330: BPHYT_RS07330 - 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase, at 1,662,550 to 1,663,470 _RS07330
Group Condition BPHYT_RS07310 BPHYT_RS07315 BPHYT_RS07320 BPHYT_RS07325 BPHYT_RS07330
phage 24hr PHYTO2 MOI 1 -0.4 -0.5 -4.0 -1.6 -0.2
phage 36hr PHYTO7 MOI 0.1 +0.3 -0.1 -4.0 -1.6 -0.4
carbon source adipate (C) -0.3 -0.1 -1.2 -3.9 +0.2
phage 36hr PHYTO6 MOI 0.1 -0.5 +0.7 -3.8 -1.4 -0.0
phage 24hr PHYTO6 MOI 1 +0.0 +0.7 -3.8 -1.4 -0.3
phage P. phyto PHYTO2 MOI 1 -0.0 +0.8 -3.7 -1.6 -0.1
phage 24hr PHYTO3 MOI 1 -0.2 +0.8 -3.8 -1.4 +0.3
phage 36hr PHYTO7 MOI 1 +0.1 -0.1 -3.6 -1.2 +0.3
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.1 -0.1 -0.3 -4.0 +0.2
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 +0.2 +0.2 -0.6 -3.9 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 -0.7 -1.0 -2.3 +0.0
phage 24hr PHYTO1 MOI 1 -0.2 +0.1 -2.4 -1.6 +0.1
vitamin With vitamins t1 +0.4 +0.2 -1.4 -3.2 +0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.2 -0.5 -0.5 -2.7 -0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB -0.0 -0.3 +0.9 -4.5 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.5 +0.3 -0.7 -3.6 -0.0
phage 36hr PHYTO2 MOI 1 -0.3 +0.2 -2.1 -1.3 +0.0
phage 24hr PHYTO2 MOI 0.1 +0.1 +0.3 -2.3 -1.4 -0.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days -0.3 +0.4 -1.7 -1.8 +0.4
phage 36hr PHYTO2 MOI 1 -0.5 +0.5 -1.1 -1.4 -0.4
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -0.2 +0.4 +0.6 -3.5 -0.2
nitrogen source Uridine (N) -0.7 -0.2 +1.0 -2.6 -0.2
nitrogen source L-Phenylalanine (N) -0.1 -0.1 +1.1 -3.4 -0.1
phage 24hr PHYTO MOI 0.1 +0.3 +0.5 -1.6 -1.5 -0.3
phage 36hr PHYTO4 MOI 1 +0.4 -0.1 -3.5 +0.4 +0.5
nitrogen source NAG (N) +0.2 -0.2 +0.8 -2.9 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +1.2 +0.2 -0.4 -3.4 +0.3
carbon source 2-Deoxy-D-ribonic lithium salt (C) -0.3 +1.0 +0.7 -1.7 +0.2
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X +0.2 +0.6 -1.0 +1.8 -0.4
phage 24hr PHYTO6 MOI 0.1 +0.0 +0.5 -0.7 +2.6 +0.2
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