Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS07260 and BPHYT_RS07265 are separated by 30 nucleotidesBPHYT_RS07265 and BPHYT_RS07270 are separated by 14 nucleotidesBPHYT_RS07270 and BPHYT_RS07275 are separated by 67 nucleotidesBPHYT_RS07275 and BPHYT_RS07280 are separated by 115 nucleotides BPHYT_RS07260: BPHYT_RS07260 - 2-aminomuconate deaminase, at 1,648,240 to 1,648,671 _RS07260 BPHYT_RS07265: BPHYT_RS07265 - 3-hydroxyanthranilate 3,4-dioxygenase, at 1,648,702 to 1,649,226 _RS07265 BPHYT_RS07270: BPHYT_RS07270 - aminocarboxymuconate-semialdehyde decarboxylase, at 1,649,241 to 1,650,263 _RS07270 BPHYT_RS07275: BPHYT_RS07275 - kynureninase, at 1,650,331 to 1,651,560 _RS07275 BPHYT_RS07280: BPHYT_RS07280 - amino acid permease, at 1,651,676 to 1,653,076 _RS07280
Group Condition BPHYT_RS07260 BPHYT_RS07265 BPHYT_RS07270 BPHYT_RS07275 BPHYT_RS07280
r2a control R2A control N.D. -0.6 N.D. -0.7 -0.4
motility outer cut, LB soft agar motility assay N.D. -0.4 N.D. -0.7 -0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days N.D. -0.8 N.D. -0.9 +0.0
phage 36hr PHYTO10 MOI 10 N.D. -0.3 N.D. -0.9 -0.4
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 N.D. -1.1 N.D. -0.7 +0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -1.4 N.D. -0.2 +0.2
phage 24hr PHYTO11 MOI 1 N.D. -0.3 N.D. -1.1 -0.0
phage 24hr PHYTO9 MOI 10 N.D. -0.5 N.D. -0.6 -0.3
phage P. phyto PHYTO2 MOI 1 N.D. +0.3 N.D. -1.6 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.5 N.D. -0.6 -1.0
phage 36hr PHYTO7 MOI 0.1 N.D. +0.1 N.D. -0.9 -0.4
nitrogen source L-Lysine (N) N.D. -1.0 N.D. -0.4 +0.3
carbon source D-Glucose 20 mM (C) N.D. -0.6 N.D. -0.5 +0.2
carbon source Malonic (C) N.D. -0.5 N.D. -0.6 +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.5 N.D. -0.6 +0.4
motility inner cut, LB soft agar motility assay N.D. +0.3 N.D. -1.0 +0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -1.1 N.D. +0.5 +0.1
phage 36hr PHYTO8 MOI 10 N.D. +0.5 N.D. -0.3 -0.6
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X N.D. +0.5 N.D. -0.2 -0.7
phage 36hr PHYTO3 MOI 0.1 N.D. +0.3 N.D. -0.9 +0.3
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. -0.6 N.D. -0.3 +0.6
phage P. phyto PHYTO2 MOI 0.1 N.D. +0.3 N.D. -0.9 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. +0.8 N.D. -0.6 +0.1
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 N.D. -0.6 N.D. +0.2 +0.9
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +1.2 N.D. -0.3 -0.1
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.2 N.D. +1.1 +0.6
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days N.D. +1.8 N.D. +0.1 +0.1
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days N.D. +1.7 N.D. +0.4 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +2.1 N.D. +0.5 -0.1
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days N.D. +2.3 N.D. +0.3 +0.4
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