Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS07210 and BPHYT_RS07215 are separated by 107 nucleotidesBPHYT_RS07215 and BPHYT_RS07220 are separated by 105 nucleotidesBPHYT_RS07220 and BPHYT_RS07225 are separated by 211 nucleotidesBPHYT_RS07225 and BPHYT_RS07230 are separated by 202 nucleotides BPHYT_RS07210: BPHYT_RS07210 - permease, at 1,638,851 to 1,639,741 _RS07210 BPHYT_RS07215: BPHYT_RS07215 - LysR family transcriptional regulator, at 1,639,849 to 1,640,754 _RS07215 BPHYT_RS07220: BPHYT_RS07220 - GCN5 family acetyltransferase, at 1,640,860 to 1,641,489 _RS07220 BPHYT_RS07225: BPHYT_RS07225 - membrane protein, at 1,641,701 to 1,641,862 _RS07225 BPHYT_RS07230: BPHYT_RS07230 - LysR family transcriptional regulator, at 1,642,065 to 1,643,042 _RS07230
Group Condition BPHYT_RS07210 BPHYT_RS07215 BPHYT_RS07220 BPHYT_RS07225 BPHYT_RS07230
nitrogen source L-Glutamic (N) -0.5 -0.7 -3.8 N.D. -0.5
phage 36hr PHYTO11 MOI 0.1 -0.4 -0.6 -2.4 N.D. +0.1
phage 24hr PHYTO9 MOI 10 -0.2 -1.0 -1.1 N.D. +0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB +0.3 -0.3 -2.1 N.D. +0.1
phage 24hr PHYTO6 MOI 10 -1.2 -0.9 +0.3 N.D. -0.1
phage 36hr PHYTO11 MOI 1 -0.8 -0.6 -0.6 N.D. +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.6 -0.7 +0.4 N.D. -0.6
phage P. phyto PHYTO2 MOI 1 +1.7 -1.2 -2.4 N.D. +0.6
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.7 +0.2 -1.7 N.D. +1.0
phage 24hr PHYTO7 MOI 10 -0.9 -0.9 +0.5 N.D. +0.4
phage 24hr PHYTO2 MOI 0.1 +0.3 +0.6 -1.9 N.D. +0.1
phage 24hr PHYTO3 MOI 1 -0.1 -0.3 -1.4 N.D. +0.9
supernatant control Vogels_fungal_media; pH 5.5 -1.0 +0.1 -1.0 N.D. +1.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.9 -0.2 -0.8 N.D. -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.4 -1.1 +0.3 N.D. +0.8
phage 36hr PHYTO2 MOI 1 -1.2 +1.2 +0.2 N.D. -0.5
carbon source D-Glucose 20 mM (C) +1.1 -0.1 -0.3 N.D. -0.9
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.7 +0.7 -1.2 N.D. +0.0
phage 36hr PHYTO1 MOI 10 -1.0 -0.3 +0.7 N.D. +1.0
phage 36hr PHYTO8 MOI 0.1 -0.8 +0.4 -0.2 N.D. +1.0
motility inner cut, LB soft agar motility assay +1.2 -0.7 -0.1 N.D. +0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +1.4 -0.2 +0.4 N.D. -0.5
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.0 -0.6 +0.1 N.D. +1.9
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +0.9 +0.4 -0.4 N.D. +0.7
carbon source 2-Deoxy-D-ribonic lithium salt (C) -0.6 +0.7 +0.6 N.D. +0.9
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -0.4 +0.7 +0.6 N.D. +1.3
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -0.2 +0.9 +0.2 N.D. +1.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.4 +0.4 +1.4 N.D. +0.1
carbon source Azelaic (C) +0.3 -0.0 +1.2 N.D. +0.8
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -0.2 +0.5 +0.5 N.D. +1.9
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