Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS07135 and BPHYT_RS07140 are separated by 9 nucleotidesBPHYT_RS07140 and BPHYT_RS07145 are separated by 297 nucleotidesBPHYT_RS07145 and BPHYT_RS07150 are separated by 304 nucleotidesBPHYT_RS07150 and BPHYT_RS07155 are separated by 282 nucleotides BPHYT_RS07135: BPHYT_RS07135 - biopolymer transporter ExbD, at 1,617,722 to 1,618,150 _RS07135 BPHYT_RS07140: BPHYT_RS07140 - hypothetical protein, at 1,618,160 to 1,619,344 _RS07140 BPHYT_RS07145: BPHYT_RS07145 - DNA-binding protein, at 1,619,642 to 1,620,217 _RS07145 BPHYT_RS07150: BPHYT_RS07150 - TonB-dependent receptor, at 1,620,522 to 1,623,266 _RS07150 BPHYT_RS07155: BPHYT_RS07155 - peptide ABC transporter substrate-binding protein, at 1,623,549 to 1,625,504 _RS07155
Group Condition BPHYT_RS07135 BPHYT_RS07140 BPHYT_RS07145 BPHYT_RS07150 BPHYT_RS07155
phage 24hr PHYTO3 MOI 10 N.D. -0.8 -2.3 -0.7 -0.1
stress Benzethonium chloride 0.0016 mM N.D. -0.1 -3.1 -0.1 -0.2
phage P. phyto corn MOI 0.1 N.D. -1.1 -1.4 -0.4 -0.4
stress Bacitracin 0.25 mg/ml N.D. -0.1 -2.6 -0.3 -0.3
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent N.D. -0.8 -1.5 -0.1 -0.7
phage P. phyto PHYTO2 MOI 1 N.D. -2.5 +0.1 -0.0 -0.5
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 N.D. -0.8 -1.3 -0.2 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. -0.8 -1.2 -0.4 +0.1
nitrogen source Putrescine (N) N.D. -0.4 -1.8 +0.1 -0.1
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days N.D. +0.4 -1.1 -0.6 -0.8
phage P. phyto PHYTO2 MOI 1 N.D. -0.9 -0.9 +0.3 -0.4
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days N.D. -1.0 -0.8 -0.3 +0.8
phage 36hr PHYTO10 MOI 10 N.D. -1.2 +1.0 -0.2 -0.6
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days N.D. -0.4 -0.7 -0.5 +1.0
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days N.D. -1.3 +0.1 -0.2 +0.9
phage 36hr PHYTO2 MOI 1 N.D. +0.9 -1.3 +0.2 -0.2
carbon source D-Glucose (C) N.D. -0.9 +0.4 +0.7 -0.5
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days N.D. -1.4 +0.5 +0.3 +0.4
phage 36hr PHYTO8 MOI 0.1 N.D. +0.7 +0.5 -0.6 -0.6
phage 24hr PHYTO1 MOI 1 N.D. +0.7 +0.7 -0.4 -0.5
phage 24hr PHYTO MOI 0.1 N.D. +1.3 +0.3 -0.0 -1.0
phage 24hr PHYTO6 MOI 10 N.D. +0.3 +1.3 +0.1 -0.7
phage 24hr PHYTO2 MOI 1 N.D. +0.6 +1.1 +0.3 -0.6
phage 24hr PHYTO3 MOI 0.1 N.D. +0.6 +1.3 -0.3 -0.2
phage 24hr PHYTO4 MOI 10 N.D. +1.0 +0.4 +0.5 -0.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB N.D. -0.3 +0.9 +0.6 +0.7
phage 36hr PHYTO2 MOI 1 N.D. +1.5 +0.7 -0.0 -0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. +1.0 +0.6 +0.6 +0.3
supernatant control Vogels_fungal_media; pH 5.5 N.D. +0.9 +1.3 -0.1 +0.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X N.D. +0.9 +1.1 +0.5 +0.6
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